The transcription factor GATA10 regulates fertility conversion of a two-line hybrid tms5 mutant rice via the modulation of UbL40 expression

J Integr Plant Biol. 2020 Jul;62(7):1034-1056. doi: 10.1111/jipb.12871. Epub 2019 Nov 6.

Abstract

The thermosensitive genic male sterile 5 (tms5) mutation causes thermosensitive genic male sterility in rice (Oryza sativa) through loss of RNase ZS1 function, which influences ubiquitin fusion ribosomal protein L40 (UbL40 ) messenger RNA levels during male development. Here, we used ATAC-seq, combined with analysis of H3K9ac and H3K4me2, to identify changes in accessible chromatin during fertility conversion of the two-line hybrid rice Wuxiang S (WXS) derived from a mutant tms5 allele. Furthermore, RNA-seq and bioinformatic analyses identified specific transcription factors (TFs) in differentially accessible chromatin regions. Among these TFs, only GATA10 targeted UbL40 . Osgata10 knockout mutations, which resulted in low expression of UbL40 and a tendency toward male fertility, confirmed that GATA10 regulated fertility conversion via the modulation of UbL40 . Meanwhile, GATA10 acted as a mediator for interactions with ERF65, which revealed that transcriptional regulation is a complex process involving multiple complexes of TFs, namely TF modules. It appears that the ERF141/MADS7/MADS50/MYB modules affect metabolic processes that control anther and pollen development, especially cell wall formation. Our analysis revealed that these modules directly or indirectly affect metabolic pathway-related genes to coordinate plant growth with proper anther development, and furthermore, that GATA10 regulates fertility conversion via the modulation of UbL40 expression.

MeSH terms

  • Base Sequence
  • Chromatin / metabolism
  • Epigenesis, Genetic
  • Gene Expression Regulation, Plant*
  • Genetic Markers
  • Genome, Plant
  • Hybridization, Genetic*
  • Meiosis / genetics
  • Models, Biological
  • Mutation / genetics*
  • Nucleotide Motifs / genetics
  • Oryza / anatomy & histology*
  • Oryza / genetics*
  • Phenotype
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Pollen / growth & development
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Transcriptome / genetics

Substances

  • Chromatin
  • Genetic Markers
  • Plant Proteins
  • RNA, Messenger
  • Transcription Factors