Salaquimonas pukyongi gen. nov., sp. nov., a novel bacterium within the family Phyllobacteriaceae

Int J Syst Evol Microbiol. 2019 Dec;69(12):3751-3756. doi: 10.1099/ijsem.0.003659.

Abstract

A novel aerobic, Gram-negative bacterial strain, RR3-28T, was isolated from a seawater recirculating aquaculture system in Busan, Republic of Korea. Cells were rod-shaped, non-motile, oxidase-positive, catalase-negative and grew optimally at 25-30 °C, pH 8.5 and 3 % (w/v) NaCl. Based on the results of phylogenetic analysis, strain RR3-28T was most closely related to Zhengella mangrovi X9-2-2T within the family Phyllobacteriaceae with 95.97 % 16S rRNA gene sequence similarity. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c, 71.1 %) and 11-methyl C18 : 1ω7c (14.4 %). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine and unidentified aminolipids. The predominant quinone was ubiquinone Q-10 and the DNA G+C content was 58.6 mol%. On the basis of its phenotypic and genotypic characteristics, strain RR3-28T represents a novel genus and species belonging to the family Phyllobacteriaceae, for which the name Salaquimonas pukyongi gen. nov., sp. nov. is proposed. The type strain of the species is RR3-28T (=KCTC 52649T=DSM 107947T).

Keywords: Phyllobacteriaceae; Salaquimonas pukyongi; new genus; new species.

MeSH terms

  • Aquaculture
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Phospholipids / chemistry
  • Phyllobacteriaceae / classification*
  • Phyllobacteriaceae / isolation & purification
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Ubiquinone / analogs & derivatives
  • Ubiquinone / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • coenzyme Q10
  • Ubiquinone Q2