Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 229 (5-6), 161-181

Expression and Potential Functions of KIF3A/3B to Promote Nuclear Reshaping and Tail Formation During Larimichthys Polyactis Spermiogenesis

Affiliations

Expression and Potential Functions of KIF3A/3B to Promote Nuclear Reshaping and Tail Formation During Larimichthys Polyactis Spermiogenesis

Jingqian Wang et al. Dev Genes Evol.

Abstract

KIF3A and KIF3B are homologous motor subunits of the Kinesin II protein family. KIF3A, KIF3B, and KAP3 form a heterotrimeric complex and play a significant role in spermatogenesis. Here, we first cloned full-length kif3a/3b cDNAs from Larimichthys polyactis. Lp-kif3a/3b are highly related to their homologs in other animals. The proteins are composed of three domains, an N-terminal head domain, a central stalk domain, and a C-terminus tail domain. Lp-kif3a/3b mRNAs were found to be ubiquitously expressed in the examined tissues, with high expression in the testis. Fluorescence in situ hybridization (FISH) was used to analyze the expression of Lp-kif3a/3b mRNAs during spermiogenesis. The results showed that Lp-kif3a/3b mRNAs had similar expression pattern and were continuously expressed during spermiogenesis. From middle spermatid to mature sperm, Lp-kif3a/3b mRNAs gradually localized to the side of the spermatid where the midpiece and tail form. In addition, we used immunofluorescence (IF) to observe that Lp-KIF3A protein co-localizes with tubulin during spermiogenesis. In early spermatid, Lp-KIF3A protein and microtubule signals were randomly distributed in the cytoplasm. In middle spermatid, however, the protein was detected primarily around the nucleus. In late spermatid, the protein migrated primarily to one side of the nucleus where the tail forms. In mature sperm, Lp-KIF3A and microtubules accumulated in the midpiece. Moreover, Lp-KIF3A co-localized with the mitochondria. In mature sperm, Lp-KIF3A and mitochondria were present in the midpiece. Therefore, Lp-KIF3A/KIF3B may be involved in spermiogenesis in L. polyactis, particularly during nuclear reshaping and tail formation.

Keywords: KIF3A/3B; Larimichthys polyactis; Nuclear reshaping; Spermiogenesis; Tail formation.

Similar articles

See all similar articles

References

    1. Nature. 2005 Jul 28;436(7050):583-7 - PubMed

References

    1. Mol Ecol Resour. 2016 May;16(3):755-68 - PubMed

References

    1. J Cell Biol. 2000 Mar 20;148(6):1255-65 - PubMed

References

    1. Mol Cell Endocrinol. 2013 Sep 5;377(1-2):44-55 - PubMed

References

    1. PLoS One. 2014 May 28;9(5):e97645 - PubMed

References

    1. Fish Physiol Biochem. 2018 Jun;44(3):769-788 - PubMed

References

    1. Curr Biol. 2010 Apr 27;20(8):697-702 - PubMed

References

    1. J Anat. 1997 May;190 ( Pt 4):563-76 - PubMed

References

    1. Genomics. 1998 Sep 1;52(2):219-22 - PubMed

References

    1. Trends Cell Biol. 2005 Sep;15(9):467-76 - PubMed

References

    1. FEBS J. 2017 Sep;284(18):2905-2931 - PubMed

References

    1. Mol Biol Rep. 2012 Apr;39(4):4207-14 - PubMed

References

    1. EMBO J. 2013 Feb 20;32(4):597-607 - PubMed

References

    1. Mol Reprod Dev. 2002 Sep;63(1):1-4 - PubMed

References

    1. Curr Opin Neurobiol. 2004 Oct;14(5):564-73 - PubMed

References

    1. Nat Rev Mol Cell Biol. 2009 Oct;10(10):682-96 - PubMed

References

    1. Biochim Biophys Acta. 2000 Mar 17;1496(1):142-50 - PubMed

References

    1. J Biol Chem. 2003 Sep 5;278(36):34211-8 - PubMed

References

    1. Biol Reprod. 1999 Apr;60(4):1047-56 - PubMed

References

    1. Hum Mol Genet. 2006 Nov 15;15(22):3280-92 - PubMed

References

    1. Curr Opin Cell Biol. 2012 Feb;24(1):31-9 - PubMed

References

    1. Biol Reprod. 1992 May;46(5):817-28 - PubMed

References

    1. Nat Cell Biol. 2005 May;7(5):474-82 - PubMed

References

    1. J Cell Biol. 2012 Oct 29;199(3):467-79 - PubMed

References

    1. J Cell Biol. 2006 Feb 27;172(5):663-9 - PubMed

References

    1. Cell Motil Cytoskeleton. 1997;38(1):29-37 - PubMed

References

    1. Anim Reprod Sci. 2017 Sep;184:59-77 - PubMed

References

    1. Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5043-8 - PubMed

References

    1. Curr Opin Cell Biol. 2012 Feb;24(1):24-30 - PubMed

References

    1. Mol Biol Cell. 1999 Oct;10(10):3081-96 - PubMed

References

    1. J Cell Sci. 2009 Apr 15;122(Pt 8):1174-83 - PubMed

References

    1. J Biol Chem. 2006 Feb 17;281(7):4094-9 - PubMed

References

    1. Nat Cell Biol. 2004 Nov;6(11):1109-13 - PubMed

References

    1. Nat Rev Neurosci. 2005 Mar;6(3):201-14 - PubMed

References

    1. J Clin Invest. 1998 Sep 15;102(6):1077-82 - PubMed

References

    1. Comp Biochem Physiol A Mol Integr Physiol. 2010 Nov;157(3):237-45 - PubMed

References

    1. Cell Tissue Res. 2017 Sep;369(3):625-640 - PubMed

References

    1. Physiol Rev. 2002 Oct;82(4):825-74 - PubMed

References

    1. Annu Rev Biophys. 2011;40:267-88 - PubMed

References

    1. Curr Biol. 2004 Nov 23;14(22):2025-32 - PubMed

References

    1. Mol Biol Rep. 2012 May;39(5):5589-98 - PubMed

Publication types

LinkOut - more resources

Feedback