In Vitro Optogenetic Characterization of Neuropeptide Release from Prefrontal Cortical Somatostatin Neurons

Nigel C Dao; Dakota F Brockway; Nicole A Crowley

doi:10.1016/j.neuroscience.2019.08.014

In Vitro Optogenetic Characterization of Neuropeptide Release from Prefrontal Cortical Somatostatin Neurons

Neuroscience. 2019 Nov 1:419:1-4. doi: 10.1016/j.neuroscience.2019.08.014. Epub 2019 Sep 2.

Authors

Nigel C Dao¹, Dakota F Brockway², Nicole A Crowley³

Affiliations

¹ Department of Biobehavioral Health, Pennsylvania State University, University Park, PA 16802.
² Department of Biobehavioral Health, Pennsylvania State University, University Park, PA 16802; Neuroscience Curriculum, Pennsylvania State University, University Park, PA 16802.
³ Department of Biobehavioral Health, Pennsylvania State University, University Park, PA 16802; Neuroscience Curriculum, Pennsylvania State University, University Park, PA 16802. Electronic address: nzc27@psu.edu.

PMID: 31487544
DOI: 10.1016/j.neuroscience.2019.08.014

Abstract

Somatostatin is a neuropeptide thought to play a role in a variety of neuropsychiatric disorders, and is important for healthy aging and behavioral resiliency. Physiological conditions underlying somatostatin peptidergic release are not well-defined. Using a combination of optogenetic and biochemical approaches in transgenic mice, we demonstrate an assay for the induction and inhibition of somatostatin release in mouse acute brain slices.

Keywords: anxiety; depression; prefrontal cortex; somatostatin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism
Mice, Transgenic
Neurons / metabolism*
Neuropeptides / metabolism*
Optogenetics / methods
Prefrontal Cortex / metabolism*
Somatostatin / metabolism*

Substances

Neuropeptides
Somatostatin