Structures of Respiratory Supercomplex I+III 2 Reveal Functional and Conformational Crosstalk

Mol Cell. 2019 Sep 19;75(6):1131-1146.e6. doi: 10.1016/j.molcel.2019.07.022. Epub 2019 Sep 3.

Abstract

The mitochondrial electron transport chain complexes are organized into supercomplexes (SCs) of defined stoichiometry, which have been proposed to regulate electron flux via substrate channeling. We demonstrate that CoQ trapping in the isolated SC I+III2 limits complex (C)I turnover, arguing against channeling. The SC structure, resolved at up to 3.8 Å in four distinct states, suggests that CoQ oxidation may be rate limiting because of unequal access of CoQ to the active sites of CIII2. CI shows a transition between "closed" and "open" conformations, accompanied by the striking rotation of a key transmembrane helix. Furthermore, the state of CI affects the conformational flexibility within CIII2, demonstrating crosstalk between the enzymes. CoQ was identified at only three of the four binding sites in CIII2, suggesting that interaction with CI disrupts CIII2 symmetry in a functionally relevant manner. Together, these observations indicate a more nuanced functional role for the SCs.

Keywords: bioenergetics; complex i; cryoEM; cytochrome bc1 complex; mitochondria; oxidative phosphorylation; oxidoreductas; protein structure; respiration; supercomplex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Crystallography, X-Ray
  • Electron Transport Complex I / chemistry*
  • Electron Transport Complex III / chemistry*
  • Mitochondria, Heart / enzymology*
  • Protein Structure, Quaternary
  • Sheep

Substances

  • Electron Transport Complex I
  • Electron Transport Complex III