Nucleotide Sequence of a Staphylococcal Plasmid Gene, Vgb, Encoding a Hydrolase Inactivating the B Components of Virginiamycin-Like Antibiotics

Plasmid. 1988 Nov;20(3):271-5. doi: 10.1016/0147-619x(88)90034-0.

Abstract

The nucleotide sequence of a 1883 bp fragment isolated from a resistance plasmid harbored by a Staphylococcus aureus clinical isolate and carrying the gene, vgb, encoding a hydrolase inactivating the B components of virginiamycin family has been determined. The sequence contains one open reading frame which extends from the ATG codon at nt 641 to a TGA codon at nt 1537 and which potentially codes for a protein of 33.035 Da. This value is in agreement with the apparent size (33 kDa) of the protein observed, in minicell extracts. Inactivation of the B components of the virginiamycin antibiotics as well as resistance to these antibiotics were expressed in a virginiamycin sensitive mutant of Escherichia coli recipient containing the gene on a high copy number plasmid.

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / metabolism
  • Base Sequence
  • Genes*
  • Genes, Bacterial*
  • Hydrolases / genetics*
  • Molecular Sequence Data
  • R Factors*
  • Restriction Mapping
  • Staphylococcus aureus / enzymology
  • Staphylococcus aureus / genetics*
  • Virginiamycin / metabolism

Substances

  • Anti-Bacterial Agents
  • Virginiamycin
  • Hydrolases

Associated data

  • GENBANK/J03313