Krykféie dicistrovirus: A novel dicistrovirus in velvety free-tailed bats from Brazil

Infect Genet Evol. 2019 Nov;75:104036. doi: 10.1016/j.meegid.2019.104036. Epub 2019 Sep 6.

Abstract

The Dicistroviridae family comprises positive single-stranded RNA viruses that are classified into Picornavirales order. These viruses are identified in arthropod hosts, including some having devastating economic consequences. Here, we described and characterized a novel nearly complete dicistrovirus genome identified in liver samples of velvety free-tailed bats (Molossus molossus) collected in June 2010 in Araçatuba city, São Paulo State, Brazil. This novel virus presents a genome of 9262 nucleotides in length and a typical dicistrovirus genome organization. Based on our phylogenetic analysis and ICTV criteria, we propose this virus as a novel species into the Triatovirus genus. Attempts of viral propagation in Vero E6 and C6/36 cell lines were unsuccessful. The novel dicistrovirus was detected only in one out of nine liver bat samples, representing for the first time an internal organ detection from a representative of this virus family.

Keywords: Dicistroviridae; Picornavirales; Picornavirus; Triatovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brazil
  • Chiroptera / virology*
  • Computational Biology / methods
  • Dicistroviridae / classification*
  • Dicistroviridae / genetics*
  • Genome, Viral
  • Phylogeny
  • Sequence Analysis, DNA
  • Whole Genome Sequencing