Interplay between the Mediterranean diet and C-reactive protein genetic polymorphisms towards inflammation in adolescents

Clin Nutr. 2020 Jun;39(6):1919-1926. doi: 10.1016/j.clnu.2019.08.016. Epub 2019 Aug 23.


Aim: From a nutrigenetics perspective, we aim to investigate the moderating role of the Mediterranean diet and each of its subgroups in the association between C-reactive protein (CRP) gene polymorphisms and CRP blood concentration in adolescents.

Methods: In 562 adolescents (13-17 y) of the European HELENA study, data was available on circulating CRP levels as inflammatory biomarker, three CRP gene SNPs (rs3093068, rs1204, rs1130864), food intake determined by a self-administered computerized 24 h-dietary recall for 2 days, and body composition. A 9-point Mediterranean diet score and each food subgroup were tested as moderator via SNP*diet interaction. Analyzes were adjusted for age, sex, puberty, adiposity and socioeconomic status.

Results: The minor allele frequencies of rs3093068 and rs1130864 SNPs (GG and TT, respectively) were associated with higher CRP concentrations, while rs1205 (CT/TT) was associated with lower CRP concentrations. There were significant interactions between rs3093068 and Mediterranean diet (B = -0.1139, p = 0.011), or the fish food subgroup (B = -0.0090, p = 0.022), so that those with the highest genetic CRP risk underwent the highest CRP attenuation by a healthier diet. Although the effect of diet and SNP was substantial, the explained variance by interaction was only 1%.

Conclusion: Greater adherence to the Mediterranean diet and particularly its fish component was associated with a lower CRP blood concentrations especially in those at highest genetic risk due to the rs3093068 SNP.

Keywords: C-reactive protein; Inflammation; Interaction; Mediterranean diet; Nutrigenomics; Single nucleotide polymorphism.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Age Factors
  • Animals
  • C-Reactive Protein / genetics*
  • Cross-Sectional Studies
  • Diet, Mediterranean*
  • Europe / epidemiology
  • Female
  • Fishes
  • Gene-Environment Interaction
  • Genetic Predisposition to Disease
  • Humans
  • Inflammation / diagnosis
  • Inflammation / epidemiology
  • Inflammation / genetics*
  • Inflammation / prevention & control
  • Male
  • Nutrigenomics
  • Nutritive Value
  • Phenotype
  • Polymorphism, Single Nucleotide*
  • Risk Assessment
  • Risk Factors
  • Seafood


  • C-Reactive Protein