Pediatric ependymoma: GNAO1, ASAH1, IMMT and IPO7 protein expression and 5-year prognosis correlation

Clin Neurol Neurosurg. 2019 Nov:186:105488. doi: 10.1016/j.clineuro.2019.105488. Epub 2019 Aug 12.


Objective: The aim of this work was to evaluate a pediatric ependymoma protein expression that may be useful as a molecular biomarker candidate for prognosis, correlated with clinical features such as age, gender, histopathological grade, ependymal tumor recurrence and patient survival.

Patients and methods: Immunohistochemistry assays were performed for GNAO1, ASAH1, IMMT, IPO7, Cyclin D1, P53 and Ki-67 proteins. Kaplan-Meier and Cox analysis were performed for age, gender, histopathological grade, relapse and survival correlation.

Results: We found that three proteins correlate with histopathological grade and relapse; two proteins correlate with survival; one protein does not correlate with any clinical feature.

Conclusion: Our results suggest that, out of the proteins analyzed, five may be considered suitable prognostic biomarkers and one may be considered a predictive biomarker for response to treatment of pediatric ependymoma.

Keywords: Infratentorial ependymoma; Pediatric ependymoma; Protein expression and clinical features; Protein expression and progression-free survival; Protein expression and survival.

MeSH terms

  • Acid Ceramidase / biosynthesis*
  • Acid Ceramidase / genetics
  • Adolescent
  • Biomarkers, Tumor / biosynthesis
  • Biomarkers, Tumor / genetics
  • Brain Neoplasms / diagnosis
  • Brain Neoplasms / genetics
  • Brain Neoplasms / metabolism*
  • Child
  • Child, Preschool
  • Cohort Studies
  • Ependymoma / diagnosis
  • Ependymoma / genetics
  • Ependymoma / metabolism*
  • Female
  • GTP-Binding Protein alpha Subunits, Gi-Go / biosynthesis*
  • GTP-Binding Protein alpha Subunits, Gi-Go / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Infant
  • Infant, Newborn
  • Karyopherins / biosynthesis*
  • Karyopherins / genetics
  • Male
  • Mitochondrial Proteins / biosynthesis*
  • Mitochondrial Proteins / genetics
  • Muscle Proteins / biosynthesis*
  • Muscle Proteins / genetics
  • Prognosis
  • Receptors, Cytoplasmic and Nuclear / biosynthesis*
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Time Factors


  • Biomarkers, Tumor
  • GNAO1 protein, human
  • IMMT protein, human
  • IPO7 protein, human
  • Karyopherins
  • Mitochondrial Proteins
  • Muscle Proteins
  • Receptors, Cytoplasmic and Nuclear
  • ASAH1 protein, human
  • Acid Ceramidase
  • GTP-Binding Protein alpha Subunits, Gi-Go