Photoprotective effect of vitamins A and E on polyamine and oxygenated free radical metabolism in hairless mouse epidermis

Biochimie. 1988 Dec;70(12):1709-13. doi: 10.1016/0300-9084(88)90028-4.

Abstract

The purpose of this study was to confirm the photoprotective effect on skin of vitamins A and E, due to inhibition of polyamine synthesis and production of free radicals. These variables were measured in the lumbar epidermis of the female hairless mouse subjected to UVA + B irradiation. Polyamines were assayed in epidermal homogenate by HPLC, and production of oxygenated free radicals was determined by spectrofluorometric assay of malonyl dialdehyde. It was determined that butyl-hydroxy-toluene and vitamin E inhibited production of free radicals (56% and 60%, respectively) and caused a significant reduction in polyamine biosynthesis (P less than 0.01), whereas the inhibitory effect of malonyl dialdehyde induced by vitamin A (30%) had no associated effect on polyamine metabolism.

MeSH terms

  • Animals
  • Butylated Hydroxytoluene / administration & dosage
  • Butylated Hydroxytoluene / pharmacology
  • Carotenoids / administration & dosage
  • Carotenoids / pharmacology
  • Epidermis / drug effects
  • Epidermis / metabolism*
  • Epidermis / radiation effects
  • Female
  • Free Radicals
  • Humans
  • Malondialdehyde / biosynthesis
  • Mice
  • Mice, Hairless
  • Oxygen / metabolism*
  • Polyamines / biosynthesis*
  • Radiation-Protective Agents / pharmacology*
  • Ultraviolet Rays
  • Vitamin A / pharmacology*
  • Vitamin E / pharmacology*
  • beta Carotene

Substances

  • Free Radicals
  • Polyamines
  • Radiation-Protective Agents
  • beta Carotene
  • Vitamin A
  • Vitamin E
  • Butylated Hydroxytoluene
  • Carotenoids
  • Malondialdehyde
  • Oxygen