Description of Biomaibacter acetigenes gen. nov., sp. nov., and proposal of Thermosediminibacterales ord. nov. containing two novel families of Tepidanaerobacteraceae fam. nov. and Thermosediminibacteraceae fam. nov

Int J Syst Evol Microbiol. 2019 Dec;69(12):3891-3902. doi: 10.1099/ijsem.0.003701.


A Gram-stain-negative, rod-shaped, chemoorganotrophic and anaerobic bacterium, strain SK-G1T, was isolated from oily sludge sampled at the Shengli oilfield in PR China. Growth occurred with 0-30 g l-1 NaCl, at 40-65 °C and at pH 6.0-8.5. The predominant fatty acids were C14 : 0 and C13 : 0, and the major cellular polar lipids were phosphatidylglycerol and phosphatidylethanolamine. No respiratory quinone was detected. The genomic G+C content was 43.9 mol%. The strain had highest 16S rRNA gene sequence similarity (93.2 % identity) to Tepidanaerobacter syntrophicus DSM 15584T. The phylogenetic, phenotypic and chemotaxonomic data showed that strain SK-G1T (=CCAM 530T=KCTC 15783T=JCM 33158T) represents a novel species of a new genus Biomaibacteracetigenes gen. nov., sp. nov. The results of phylogenetic and phylogenomic analyses indicated that the genera Biomaibacter, Caldanaerovirga, Fervidicola, Tepidanaerobacter, Thermosediminibacter, Thermovenabulum and Thermovoraxformed a clade with high bootstrap support distinguishing to other taxon within the order Thermoanaerobacterales. This clade is proposed as Thermosediminibacterales ord. nov. and includes Tepidanaerobacteraceae fam. nov. and Thermosediminibacteraceae fam. nov. Emended descriptions of the order Thermoanaerobacterales and family Syntrophomonadaceae are also provided.

Keywords: Biomaibacter acetigenes; Tepidanaerobacteraceae; Thermoanaerobacterales; Thermosediminibacteraceae; Thermosediminibacterales; phylogenomic analysis.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Gram-Negative Anaerobic Straight, Curved, and Helical Rods / classification*
  • Gram-Negative Anaerobic Straight, Curved, and Helical Rods / isolation & purification
  • Oil and Gas Fields / microbiology*
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sewage / microbiology*


  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Sewage