Mogroside V improves porcine oocyte in vitro maturation and subsequent embryonic development

Theriogenology. 2020 Jan 1;141:35-40. doi: 10.1016/j.theriogenology.2019.09.010. Epub 2019 Sep 6.

Abstract

Oocyte in vitro maturation (IVM) plays a pivotal role in in vitro embryo production. However, the efficiency of IVM is still low and needs to be further improved. In the present study, we evaluated the beneficial effects of mogroside V, an extract derived from Siraitia grosvenorii, on oocyte IVM. Porcine cumulus-oocyte complexes were cultured in IVM medium supplemented or not supplemented with mogroside V for 40 h. We found that mogroside V supplementation increased the percentage of oocyte first polar body extrusion and improved subsequent blastocyst formation after parthenogenetic activation. Furthermore, mogroside V reduced the levels of reactive oxygen species (ROS) and increased the mRNA expression of oxidative stress-related genes (SOD, CAT and SIRT1). Moreover, mogroside V supplementation enhanced the mitochondrial content, mtDNA copy number, mitochondrial membrane potential (ΔΨm), ATP generation, and the relative mRNA expression of mitochondria-related genes (PGC-1α and TFAM). In summary, our findings demonstrate that mogroside V supplementation reduces intracellular ROS levels and enhances mitochondrial function to promote porcine oocyte IVM.

Keywords: Embryo development; Mitochondria; Mogroside V; Oocyte in vitro maturation; ROS.

MeSH terms

  • Animals
  • DNA, Mitochondrial
  • Embryo Culture Techniques / veterinary*
  • Embryonic Development / drug effects*
  • Fertilization in Vitro / veterinary
  • In Vitro Oocyte Maturation Techniques / veterinary*
  • Mitochondria / physiology
  • Oocytes / drug effects*
  • Oocytes / metabolism
  • Reactive Oxygen Species / metabolism
  • Swine / physiology*
  • Triterpenes / pharmacology*

Substances

  • DNA, Mitochondrial
  • Reactive Oxygen Species
  • Triterpenes
  • mogroside V