Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

Nicola Isabelle Kols; Heli Aatola; Ville Peltola; Man Xu; Zaiga Nora-Krukle; Klaus Hedman; Aurelija Zvirbliene; Hanna Toivola; Tytti Vuorinen; Juha M Koskinen; Andrea H L Bruning; Andreas Christensen; Maria Söderlund-Venermo; Janne O Koskinen

doi:10.1016/j.jcv.2019.09.003

Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

J Clin Virol. 2019 Nov:120:17-19. doi: 10.1016/j.jcv.2019.09.003. Epub 2019 Sep 4.

Authors

Affiliations

¹ Department of Medical Microbiology, Clinic of Laboratory Medicine, St. Olavs Hospital HF, Trondheim University Hospital, 7006, Trondheim, Norway; Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, 7491, Trondheim, Norway. Electronic address: nicola.isabelle.kols@stolav.no.
² ArcDia International Oy Ltd, 20521, Turku, Finland.
³ Department of Paediatrics and Adolescent Medicine, Turku University Hospital and University of Turku, 20521, Turku, Finland.
⁴ Department of Virology, University of Helsinki, and Helsinki University Hospital, 00290, Helsinki, Finland.
⁵ Department of Virology, University of Helsinki, and Helsinki University Hospital, 00290, Helsinki, Finland; Institute of Microbiology and Virology, Riga Stradins University, LV-1067, Riga, Latvia.
⁶ Department of Virology, University of Helsinki, and Helsinki University Hospital, 00290, Helsinki, Finland; Helsinki University Hospital, 00029, Helsinki, Finland.
⁷ Department of Immunology and Cell Biology, Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10223, Vilnius, Lithuania.
⁸ Department of Clinical Microbiology, Turku University Hospital and Department of Virology, University of Turku, 20521, Turku, Finland.
⁹ Department of Pediatric Infectious Diseases, Emma Children's Hospital, Academic Medical Center, 1105, Amsterdam, the Netherlands.
¹⁰ Department of Medical Microbiology, Clinic of Laboratory Medicine, St. Olavs Hospital HF, Trondheim University Hospital, 7006, Trondheim, Norway; Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, 7491, Trondheim, Norway.

Abstract

Background: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA.

Objective: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection.

Study design: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR.

Results and conclusion: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.

Keywords: Diagnosis; Human bocavirus 1; Parvovirus; Pediatric infection; Polymerase chain reaction; Rapid antigen detection assay; Respiratory tract infection.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Viral / metabolism*
Automation
Child
DNA, Viral / genetics
Female
Genotype
Human bocavirus / genetics*
Human bocavirus / immunology*
Humans
Male
Parvoviridae Infections / diagnosis*
Parvoviridae Infections / immunology
Phenotype
Point-of-Care Systems
RNA, Messenger / genetics*
RNA, Viral / genetics
Respiratory Tract Infections / immunology
Respiratory Tract Infections / virology*
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Viral Load

Substances

Antigens, Viral
DNA, Viral
RNA, Messenger
RNA, Viral