Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 9 (1), 147

Effect of Different Combinations of Antibiotics on Fruit Quality and Antioxidant Defense System in Huanglongbing Infected Kinnow Orchards


Effect of Different Combinations of Antibiotics on Fruit Quality and Antioxidant Defense System in Huanglongbing Infected Kinnow Orchards

Sajjad Hussain et al. AMB Express.


Huanglongbing (HLB), also known as citrus greening disease, is the most devastating disease of citrus across the world, caused by the phloem limited fastidious bacterium 'Candidatus Liberibacter spp.'. This research was conducted on HLB infected 10-year-old Kinnow orchard located at Multan, Pakistan. Different classes of antibiotics in various combinations were applied on HLB-infected trees. The antibiotic treatments were applied before flowering in February, during fruit setting in April and at fruit growth stage in June. The different antibiotics combinations used were Ampicillin sodium + Rifampicin, Cefalexin + Rifampicin, Ampicillin sodium + Cefalexin, Ampicillin sodium + Cefalexin + Rifampicin and Control (distilled water). Different fruit qualitative and quantitative attributes were examined. The application of antibiotics significantly decreased 2-11% in flower, June and pre-harvest drops as compared to control. Further, antibiotics increased fruit weight and yield by five times while the juice content, total soluble solids, ripening index, total sugars, phenolic and vitamin C content were also increased in fruits. In addition, total soluble proteins, peroxidase and catalase activities were increased in fruits harvested from antibiotic treated plants compared to control, however the superoxidase dismutase activity was decreased in fruits of antibiotic treated plants. Finally, it is concluded that application of different antibiotics combinations helps in improving the fruit yield and different quality attributes of HLB infected Kinnow trees.

Keywords: Antioxidant activity; Citrus greening disease; Fruit drop; Fruit quality; Fruit yield.

Conflict of interest statement

The authors declare that they have no competing interests.


Fig. 1
Fig. 1
PCR product of 1160 bp amplified with primers OI1/OI2 on 1.5% agarose gel of infected Kinnow with 100 bp marker
Fig. 2
Fig. 2
PCR product of 703 bp amplified with primers A2, J5 on 1.5% agarose gel. 1–5 from infected Kinnow leaves positive control (+ive), negative control (−ve) and M (100 bp DNA ladder)

Similar articles

See all similar articles

Cited by 1 article


    1. Baldwin E, Plotto A, Manthey J, McCollum G, Bai J, Irey M, Cameron R, Luzio G. Effect of Liberibacter infection (Huanglongbing disease) of citrus on orange fruit physiology and fruit/fruit juice quality: chemical and physical analyses. J Agric Food Chem. 2009;58(2):1247–1262. doi: 10.1021/jf9031958. - DOI - PubMed
    1. Bassanezi RB, Montesino LH, Stuchi ES. Effects of huanglongbing on fruit quality of sweet orange cultivars in Brazil. Eur J Plant Pathol. 2009;125(4):565. doi: 10.1007/s10658-009-9506-3. - DOI
    1. Bové JM. Huanglongbing: a destructive, newly-emerging, century-old disease of citrus. J Plant Pathol. 2006;88:7–37.
    1. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1–2):248–254. doi: 10.1016/0003-2697(76)90527-3. - DOI - PubMed
    1. Canales E, Coll Y, Hernández I, Portieles R, García MR, López Y, Aranguren M, Alonso E, Delgado R, Luis M. ‘Candidatus Liberibacter asiaticus’, causal agent of citrus Huanglongbing, is reduced by treatment with Brassinosteroids. PLoS ONE. 2016;11(1):e0146223. doi: 10.1371/journal.pone.0146223. - DOI - PMC - PubMed

LinkOut - more resources