Direct Detection and Quantification of Neoantigens

Cancer Immunol Res. 2019 Nov;7(11):1748-1754. doi: 10.1158/2326-6066.CIR-19-0107. Epub 2019 Sep 16.


Many immunotherapeutic approaches under development rely on T-cell recognition of cancer-derived peptides bound to human leukocyte antigen molecules on the cell surface. Direct experimental demonstration that such peptides are processed and bound is currently challenging. Here, we describe a method that meets this challenge. The method entailed an optimized immunoprecipitation protocol coupled with two-dimensional chromatography and mass spectrometry. The ability to detect and quantify minute amounts of predefined antigens should be useful both for basic research in tumor immunology and for the development of rationally designed cancer vaccines.

MeSH terms

  • Animals
  • Antigens, Neoplasm / analysis
  • Antigens, Neoplasm / genetics
  • Antigens, Neoplasm / metabolism*
  • COS Cells
  • Cell Line, Tumor
  • Chlorocebus aethiops
  • Epitopes, T-Lymphocyte / metabolism
  • HLA Antigens / metabolism
  • Humans
  • Immunoprecipitation
  • Mass Spectrometry
  • Mutation
  • Neoplasms / immunology*
  • Neoplasms / metabolism
  • Peptides / metabolism


  • Antigens, Neoplasm
  • Epitopes, T-Lymphocyte
  • HLA Antigens
  • Peptides