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, 34 (7), e201900706

Protective Roles of Pyracantha Fortuneana Extract on Acute Renal Toxicity Induced by Cadmium Chloride in Rats

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Protective Roles of Pyracantha Fortuneana Extract on Acute Renal Toxicity Induced by Cadmium Chloride in Rats

Yixin Ke et al. Acta Cir Bras.

Abstract

Purpose: To investigate the protective roles of pyracantha fortune fruit extract (PFE) on acute renal toxicity induced by cadmium chloride (CdCl2) in rats.

Methods: Rats were pretreated with PFE and consecutively injected with CdCl2 (6.5 mg/kg) for 5 days.

Results: The concentration of Cd, kidney weight, malondialdehyde (MDA), and nitric oxide (NO) production were remarkably increased in CdCl2 group as well as the levels of plasma uric acid, urea, and creatinine (P < 0.001). However, the body weight and glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione peroxidase (GR) levels were markedly reduced by CdCl2 treatment (P < 0.001). Histological manifestations of renal tissue showed severely adverse changes. Moreover, CdCl2 treatment significantly decreased the B-cell lymphoma-2 (Bcl-2) expression while increased the Bcl-2-Associated X Protein (Bax), tumor necrosis factor-α (TNF-α) expression (P < 0.001). Additionally, the expression of Nrf2/Keap 1 related proteins Keap-1 gained a significant increase (P < 0.001), whereas the Nrf2, HO-1, γ-GCS, GSH-Px and NQO1 expression decreased by CdCl2 treatment (P < 0.05). These rats were pretreated with PFE to improve the changes caused by CdCl2 treatment.

Conclusion: PFE could protect the kidney against acute renal toxicity induced by CdCl2.

Conflict of interest statement

Conflict of interest: none

Figures

Figure 1
Figure 1. The effects of PFE on kidney tissues of rats treated with CdCl2. (A) Effect of PFE on the accumulation of Cd in renal tissue, kidney weights, body weights, uric acid, urea, and creatinine plasma levels of rats treated with CdCl2, ***P < 0.001 vs. Con group, *P < 0.05, ***P < 0.001 vs. CdCl2 group. (B) Histological characteristics of glomerular and tubules in control rats, CdCl2-treated rats, PFE-treated rats and the rats where PFE was administered before CdCl2, vacuoles (black arrows), inflammation and extensive degeneration (black star) (Scan bar, x400).
Figure 2
Figure 2. Effects of PFE pretreatment on oxidative stress markers in rats treated with CdCl2. Analysis of MDA content, nitric oxide level, the SOD, CAT, GPx and GR activities in the renal tissue of control rats, CdCl2-treated rats, PFE-treated rats and the rats where PFE was administered before CdCl2, ***P < 0.001 vs. Con group, **P< 0.01, ***P< 0.001 vs. CdCl2 group.
Figure 3
Figure 3. The effects of PFE on the Bcl-2, Bax, and TNF-α genes expression in the renal tissue of rats treated with CdCl2. (A) The Bcl-2, Bax, and TNF-α mRNA expression was evaluated by qRT-PCR, ***P< 0.001 vs. Con group, *P < 0.05, ***P < 0.001 vs. CdCl2 group. (B) The Bcl-2, Bax and TNF-α protein expression was evaluated by western blot, ***P < 0.001 vs. Con group, *P < 0.05, **P < 0.01 vs. CdCl2 group.
Figure 4
Figure 4. The effects of PFE on Nrf2-Keap1 signaling pathway in rats treated with CdCl2. The protein expression of Nrf2, Keap1, HO-1, γ-GCS, GSH-Px and NQO1 was evaluated by western blot, *P < 0.05, ***P < 0.001 vs. Con group, **P < 0.01, ***P < 0.001 vs. CdCl2 group.

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