Cardiac expression of the microsomal triglyceride transport protein protects the heart function during ischemia

J Mol Cell Cardiol. 2019 Dec;137:1-8. doi: 10.1016/j.yjmcc.2019.09.003. Epub 2019 Sep 15.

Abstract

Aims: The microsomal triglyceride transport protein (MTTP) is critical for assembly and secretion of apolipoprotein B (apoB)-containing lipoproteins and is most abundant in the liver and intestine. Surprisingly, MTTP is also expressed in the heart. Here we tested the functional relevance of cardiac MTTP expression.

Materials and methods: We combined clinical studies, advanced expression analysis of human heart biopsies and analyses in genetically modified mice lacking cardiac expression of the MTTP-A isoform of MTTP.

Results: Our results indicate that lower cardiac MTTP expression in humans is associated with structural and perfusion abnormalities in patients with ischemic heart disease. MTTP-A deficiency in mice heart does not affect total MTTP expression, activity or lipid concentration in the heart. Despite this, MTTP-A deficient mice displayed impaired cardiac function after a myocardial infarction. Expression analysis of MTTP indicates that MTTP expression is linked to cardiac function and responses in the heart.

Conclusions: Our results indicate that MTTP may play an important role for the heart function in conjunction to ischemic events.

Keywords: Expression; Function; Heart; MTTP; Polymorphism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiotonic Agents / metabolism*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Female
  • Gene Expression Regulation
  • Heart / physiopathology*
  • Humans
  • Lipid Metabolism
  • Liver / metabolism
  • Male
  • Mice, Knockout
  • Middle Aged
  • Myocardial Infarction / diagnostic imaging
  • Myocardial Infarction / genetics
  • Myocardial Infarction / physiopathology
  • Myocardial Ischemia / genetics
  • Myocardial Ischemia / physiopathology*
  • Polymorphism, Single Nucleotide / genetics
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • Cardiotonic Agents
  • Carrier Proteins
  • Protein Isoforms
  • RNA, Messenger
  • microsomal triglyceride transfer protein