Synergism Effects of Ursolic Acid Supplementation on the Levels of Irisin, C-reactive Protein, IL-6, and TNF-α During High-intensity Resistance Training in Low Activity Men

Cardiovasc Hematol Disord Drug Targets. 2020;20(2):138-144. doi: 10.2174/1871529X19666190918144727.


Background: Ursolic Acid (UA) is a pentacyclic triterpenoid carboxylic acid which is extracted from plants. UA may enhance the effect of Resistance Training (RT) in human.

Objective: Current research was designed to show the effect of High-Intensity Resistance Training (HIRT) in the presence or absence of UA on the serum levels of irisin, CRP, IL-6 and TNF-α in the low activity men.

Methods: The study included twenty-two healthy male HIRT with placebo, supplementation, and HIRT in the presence of UA supplementation. The two groups received eight-week intervention including 2 sets of 8 exercises, with 8~10 repetitions at 70~75% of 1 repetition maximum and a 2 min rest interval between sets, performed 3 times/week. Placebo or UA orally was evaluated as 1 capsule 3 times/day during 8 weeks. The subsequent factors were measured post- and preintervention: C-Reactive Protein (CRP), Irisin, Tumor Necrotic Factor (TNF-α) and Interleukin-6 (IL-6).

Results: UA supplementation significantly increased the plasma levels of irisin in the HIRT+UA group versus the HIRT+P group (p<0.05). UA treatment also dramatically decreased the plasma levels of CRP, IL-6, and TNF-α in the HIRT+UA group versus the HIRT+P group (p<0.05).

Conclusion: The current data showed that UA-induced an increase in serum irisin and reduction of CRP, IL-6, and TNF-α may have beneficial effects as a chemical for increasing of the effects of HIRT in low activity men.

Keywords: C-reactive protein; IL-6; Irisin; TNF-α; Ursolic acid; resistance training..

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • C-Reactive Protein / metabolism*
  • Dietary Supplements
  • Fibronectins / blood*
  • Humans
  • Interleukin-6 / blood*
  • Male
  • Plant Extracts / pharmacology
  • Resistance Training*
  • Triterpenes / pharmacology*
  • Tumor Necrosis Factor-alpha / blood*
  • Ursolic Acid


  • FNDC5 protein, human
  • Fibronectins
  • IL6 protein, human
  • Interleukin-6
  • Plant Extracts
  • Triterpenes
  • Tumor Necrosis Factor-alpha
  • C-Reactive Protein