Molecular cloning and expression of mouse placental lactogen I complementary deoxyribonucleic acid

Mol Endocrinol. 1987 Nov;1(11):767-76. doi: 10.1210/mend-1-11-767.


The mouse midpregnancy lactogen or placental lactogen I (mPL-I) is encoded by a 1.0-kilobase mRNA that appears transiently during gestation, with maximal amounts accumulating in the placenta at day 10 of pregnancy. Several cDNA clones for mPL-I have been isolated from a lambda gt11 expression library constructed from day 10-placental RNA. The cDNA sequence indicates that mPL-I is synthesized as a 224 amino acid precursor, and is secreted as a 194 amino acid glycosylated hormone. The deduced amino acid sequence of mPL-I is highly homologous to the known members of the PRL family in the mouse, and hybridization analysis indicates that the mouse genome contains several mPL-I genes. Introduction of the mPL-I cDNA in an expression vector into cultured mouse cells results in the synthesis and secretion of glycosylated mPL-I protein that is recognized by anti-mPL-I antiserum and is biologically active.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • Female
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Placenta / chemistry
  • Placental Lactogen / biosynthesis
  • Placental Lactogen / genetics*
  • Precipitin Tests
  • Pregnancy
  • Protein Biosynthesis / physiology
  • RNA, Messenger / metabolism
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid


  • RNA, Messenger
  • DNA
  • Placental Lactogen