[Studies on KU-1 and KU-7 cells as an in vitro model of human transitional cell carcinoma of urinary bladder]

Hum Cell. 1988 Mar;1(1):78-83.
[Article in Japanese]


Cellular characteristics of KU-1 and KU-7 cells after a long term in culture were evaluated for eligibility of in vitro model of human transitional cell carcinoma of the urinary bladder. The KU-7 cells derived from superficial papillary tumor showed small, polygonal and homogeneous cells, while the KU-1 cells derived from a broad basic invasive carcinoma showed a variety in size as well as piling up tendency. The adhesiveness of these cells in culture was examined and a significant difference in their cellular structures, a thick multilayered mass in KU-1 while a thin flat spreading in KU-7, was found by each rotating culture and culture on collagen sponge matrix. KU-7 cells cultured without sera responded to transforming growth factor (TGF-alpha) but KU-1 cells did not show any response to the factor. These findings indicate that KU-7 and KU-1 cells have maintained some basic characteristics of papillary tumors and infiltrating carcinomas, respectively.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Carcinoma, Transitional Cell / pathology*
  • Carcinoma, Transitional Cell / ultrastructure
  • Microscopy, Electron
  • Models, Biological
  • Tumor Cells, Cultured*
  • Urinary Bladder Neoplasms / pathology*
  • Urinary Bladder Neoplasms / ultrastructure