The effects of serum levels, and alterations in the genes of binding protein and receptor of vitamin D on gastric cancer

Mol Biol Rep. 2019 Dec;46(6):6413-6420. doi: 10.1007/s11033-019-05088-9. Epub 2019 Sep 23.

Abstract

Due to many biological cell functions of vitamin D including regulation of cell survival, proliferation and differentiation, the metabolism of itself gains importance in the development of several types of cancer. This case-control study was designed to evaluate the risk of gastric cancer development in terms of VDR rs2228570 & rs731236, and VDBP rs7041 polymorphisms, and serum levels of vitamin D. The study consists of 77 gastric cancer patients and 84 healthy individuals. VDR and VDBP gene polymorphisms and vitamin D levels were determined by using PCR-RFLP and HPLC methods. The distribution of VDR or VDBP gene variants were not different in study groups. The serum level of 25-hydroxyvitamin D was significantly lower in gastric cancer patients versus controls (16 ± 6 → 11 ± 6 ng/ml) in which male patients have higher levels than females. Although the whole study population lacks normal levels of 25-hydroxyvitamin D, it was found that the risk of the development of gastric cancer was approximately fourfold higher in cases with severe vitamin D (< 10 ng/ml) deficiency. Our results indicate that VDR rs731236 & rs2228570 or VDBP rs7041 polymorphisms were not risk factors for the development of gastric cancer individually, however, lower serum levels of vitamin D may be a contributory risk for both predisposition and development of gastric cancer.

Keywords: Gastric cancer; Polymorphism; VDBP; VDR; Vitamin D.

MeSH terms

  • Case-Control Studies
  • Female
  • Genetic Predisposition to Disease
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Grading
  • Polymorphism, Single Nucleotide*
  • Receptors, Calcitriol / genetics*
  • Sex Characteristics
  • Stomach Neoplasms / blood
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / pathology*
  • Vitamin D / blood*
  • Vitamin D-Binding Protein / genetics*

Substances

  • GC protein, human
  • Receptors, Calcitriol
  • VDR protein, human
  • Vitamin D-Binding Protein
  • Vitamin D