Presence and Diversity of Extended-Spectrum Cephalosporin Resistance Among Escherichia coli from Urban Wastewater and Feedlot Cattle in Alberta, Canada

Abstract

A recent preliminary study from our group found that extended-spectrum cephalosporin-resistance determinants can be detected in the majority of composite fecal samples collected from Alberta feedlot cattle. Most notably, bla_CTX-M genes were detected in 46.5% of samples. Further isolate characterization identified bla_CTX-M-15 and bla_CTX-M-27, which are widespread in bacteria from humans. We hypothesized that Escherichia coli of human and beef cattle origins share the same pool of bla_CTX-M genes. In this study, we aimed to assess and compare the genomic profiles of a larger collection of bla_CTX-M-positive E. coli recovered from fecal composite samples from Canadian beef feedlot cattle and human wastewater through whole-genome sequencing. The variants bla_CTX-M-55, bla_CTX-M-32, bla_CTX-M-27, bla_CTX-M-15, and bla_CTX-M-14 were found in both urban wastewater and cattle fecal isolates. Core genome multilocus sequence typing showed little similarity between the fecal and wastewater isolates. Thus, if the dissemination of genes between urban wastewater and feedlot cattle occurs, it does not appear to be related to the expansion of specific clonal lineages. Further investigations are warranted to assemble and compare plasmids carrying these genes to better understand the modalities and directionality of transfer.

Keywords: CTX-M; Escherichia coli; beef cattle; extended-spectrum cephalosporin resistance; wastewater; whole-genome sequencing.