Clinical response of a patient with diffuse histiocytic lymphoma to adoptive chemoimmunotherapy using cyclophosphamide and alloactivated haploidentical lymphocytes. A case report and phase I trial

Cancer. 1985 Feb 1;55(3):552-60. doi: 10.1002/1097-0142(19850201)55:3<552::aid-cncr2820550314>;2-2.


Adoptive chemoimmunotherapy has cured experimentally induced tumors in animals, but its clinical use has been limited. Six patients were treated with refractory neoplasms in a Phase I study with cyclophosphamide (CPM) and alloactivated haploidentical lymphocytes. Patients received an immunosuppressive dose of CPM (800 mg/m2) followed by haploidentical lymphocytes primed in vitro with alloantigens in mixed lymphocyte culture (MLC). One week later patients received a second infusion of alloactivated lymphocytes expanded in T-cell growth factor (TCGF). The total number of cells given to each patient progressively increased, with a single patient receiving 35.5 X 10(9) cells. Transient febrile responses and delayed-type hypersensitivity reactions at the intravenous sites were the only toxicities noted. A complete clinical response lasting 12 weeks was seen in a single patient with diffuse histiocytic lymphoma. Our experience indicates that adoptive chemoimmunotherapy can be given to patients safely and merits further clinical testing.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blood Transfusion
  • Cells, Cultured
  • Combined Modality Therapy
  • Cyclophosphamide / adverse effects
  • Cyclophosphamide / therapeutic use*
  • Evaluation Studies as Topic
  • Female
  • HLA Antigens / immunology
  • Haploidy
  • Humans
  • Immunotherapy / methods
  • Indium
  • Interleukin-2 / immunology
  • Isoantigens / immunology
  • Leukapheresis
  • Lymphocyte Activation
  • Lymphocyte Culture Test, Mixed
  • Lymphocyte Transfusion*
  • Lymphocytes / immunology
  • Lymphocytes / ultrastructure
  • Lymphoma, Large B-Cell, Diffuse / diagnostic imaging
  • Lymphoma, Large B-Cell, Diffuse / therapy*
  • Male
  • Middle Aged
  • Radioisotopes
  • Radionuclide Imaging


  • HLA Antigens
  • Interleukin-2
  • Isoantigens
  • Radioisotopes
  • Indium
  • Cyclophosphamide