Preliminary Study: Comparison of Antioxidant Activity of Cannabidiol (CBD) and α-Tocopherol Added to Refined Olive and Sunflower Oils

Molecules. 2019 Sep 26;24(19):3485. doi: 10.3390/molecules24193485.

Abstract

This study evaluates the antioxidant activity of cannabidiol (CBD), added to model systems of refined olive (ROO) and sunflower (SO) oils, by measuring the peroxide value, oxidative stability index (OSI), electron spin resonance (ESR) forced oxidation, and DPPH assays. Free acidity, a parameter of hydrolytic rancidity, was also examined. CBD was compared using the same analytical scheme with α-tocopherol. CBD, compared to α-tocopherol, showed a higher scavenging capacity, measured by DPPH assay, but not better oxidative stability (OSI) of the oily systems considered. In particular, α-tocopherol (0.5%) showed an antioxidant activity only in SO, registered by an increase of more than 30% of the OSI (from 4.15 ± 0.07 to 6.28 ± 0.11 h). By ESR-forced oxidation assay, the concentration of free radicals (μM) in ROO decreased from 83.33 ± 4.56 to 11.23 ± 0.28 and in SO from 19.21 ± 1.39 to 6.90 ± 0.53 by adding 0.5% α-tocopherol. On the contrary, the addition of 0.5% CBD caused a worsening of the oxidative stability of ROO (from 23.58 ± 0.32 to 17.28 ± 0.18 h) and SO (from 4.93 ± 0.04 to 3.98 ± 0.04 h). Furthermore, 0.5% of CBD did not lower dramatically the concentration of free radicals (μM) as for α-tocopherol, which passed from 76.94 ± 9.04 to 72.25 ± 4.13 in ROO and from 17.91 ± 0.95 to 16.84 ± 0.25 in SO.

Keywords: antioxidant activity; cannabidiol (CBD); free radicals; lipid oxidation; oxidative stability; α-tocopherol.

MeSH terms

  • Antioxidants / chemistry*
  • Antioxidants / pharmacology*
  • Cannabidiol / chemistry*
  • Free Radicals / antagonists & inhibitors
  • Lipid Peroxidation / drug effects
  • Olive Oil / chemistry
  • Olive Oil / pharmacology*
  • Oxidation-Reduction / drug effects
  • Sunflower Oil / chemistry
  • Sunflower Oil / pharmacology*
  • alpha-Tocopherol / chemistry*

Substances

  • Antioxidants
  • Free Radicals
  • Olive Oil
  • Sunflower Oil
  • Cannabidiol
  • alpha-Tocopherol