Calcium Phosphate-Mediated Transfection of Cells with High-Molecular-Weight Genomic DNA

Cold Spring Harb Protoc. 2019 Oct 1;2019(10). doi: 10.1101/pdb.prot095448.

Abstract

This protocol describes a calcium phosphate-mediated transfection method for use with high-molecular-weight genomic DNAs.

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • CHO Cells / drug effects
  • CHO Cells / metabolism
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / metabolism*
  • Calcium Phosphates / pharmacology
  • Cricetinae
  • Cricetulus
  • DNA / chemistry
  • DNA / genetics*
  • DNA / metabolism
  • Genome / genetics
  • Glycerol / chemistry
  • Glycerol / metabolism
  • Glycerol / pharmacology
  • Molecular Weight
  • Plasmids / genetics
  • Transfection / instrumentation
  • Transfection / methods*

Substances

  • Calcium Phosphates
  • DNA
  • calcium phosphate
  • Glycerol