Abstract
Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured cells. Here, we briefly review the use of calcium phosphate-DNA coprecipitates for transfection.
© 2019 Cold Spring Harbor Laboratory Press.
MeSH terms
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Animals
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Calcium Phosphates / chemistry
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Calcium Phosphates / metabolism*
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Calcium Phosphates / pharmacology
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Cell Line
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Chemical Precipitation / drug effects
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Chloroquine / chemistry
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Chloroquine / metabolism
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Chloroquine / pharmacology
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DNA / chemistry
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DNA / genetics*
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DNA / metabolism
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Eukaryotic Cells / cytology
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Eukaryotic Cells / drug effects
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Eukaryotic Cells / metabolism*
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HeLa Cells
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Humans
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Hydrogen-Ion Concentration
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Mice
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Molecular Weight
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NIH 3T3 Cells
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Transfection / methods*
Substances
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Calcium Phosphates
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Chloroquine
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DNA
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calcium phosphate