Transfection of Mammalian Cells with Calcium Phosphate-DNA Coprecipitates

Cold Spring Harb Protoc. 2019 Oct 1;2019(10). doi: 10.1101/pdb.top096255.

Abstract

Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured cells. Here, we briefly review the use of calcium phosphate-DNA coprecipitates for transfection.

Publication types

  • Review

MeSH terms

  • Animals
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / metabolism*
  • Calcium Phosphates / pharmacology
  • Cell Line
  • Chemical Precipitation / drug effects
  • Chloroquine / chemistry
  • Chloroquine / metabolism
  • Chloroquine / pharmacology
  • DNA / chemistry
  • DNA / genetics*
  • DNA / metabolism
  • Eukaryotic Cells / cytology
  • Eukaryotic Cells / drug effects
  • Eukaryotic Cells / metabolism*
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Mice
  • Molecular Weight
  • NIH 3T3 Cells
  • Transfection / methods*

Substances

  • Calcium Phosphates
  • Chloroquine
  • DNA
  • calcium phosphate