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. 2019 Oct 1;9(1):14052.
doi: 10.1038/s41598-019-49941-0.

Biological and antibacterial properties of the micro-nanostructured hydroxyapatite/chitosan coating on titanium

Baoe Li  1 , Xiaomei Xia  1 , Miaoqi Guo  1 , Yu Jiang  1 , Yu Li  1 , Zhiyuan Zhang  1 , Shimin Liu  2 , Haipeng Li  1 , Chunyong Liang  3 , Hongshui Wang  1
Affiliations
Free PMC article

Biological and antibacterial properties of the micro-nanostructured hydroxyapatite/chitosan coating on titanium

Baoe Li et al. Sci Rep. .
Free PMC article

Abstract

Titanium (Ti) is the widely used implant material in clinic, however, failures still frequently occur due to its bioinertness and poor antibacterial property. To improve the biological and antibacterial properties of Ti implants, micro-nanostructured hydroxyapatite (HA) coating was prepared on Ti surface by micro-arc oxidation (MAO), and then the antibacterial agent of chitosan (CS) was loaded on the HA surface through dip-coating method. The results showed that the obtained HA/CS composite coating accelerated the formation of apatite layer in SBF solution, enhanced cell adhesion, spreading and proliferation, and it also inhibited the bacterial growth, showing improved biological and antibacterial properties. Although, with the increased CS amount, the coverage of HA coating would be enlarged, resulting in depressed biological property, however, the antibacterial property of the composite coating was enhanced, and the cytotoxicity about CS was not detected in this work. In conclusion, the HA/CS coating has promising application in orthopedics, dentistry and other biomedical devices.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Surface morphologies of the HA and HA/CS composite coating: HA (a), HA/0.2CS (b), HA/0.5CS (c), HA/1CS (d), HA/2CS (e), HA/6CS (f).
Figure 2
Figure 2
XRD patterns of the HA and HA/CS coatings: HA (a), HA/0.2CS (b), HA/0.5CS (c), HA/1CS (d), HA/2CS (e), HA/4CS (f), HA/6CS (g).
Figure 3
Figure 3
FT-IR spectra of the HA, CS and HA/0.2CS composite coating.
Figure 4
Figure 4
TGA curves of the different samples.
Figure 5
Figure 5
Surface roughness (a) and water contact angles (b) of the corresponding composite coatings, data expressed as mean ± SD (n = 3), *a statistical significance compared to the HA group (P < 0.05).
Figure 6
Figure 6
Surface morphologies of HA (a), HA/0.2CS (b), HA/6CS (c) coatings after immersion in SBF for 2 weeks, and corresponding XRD patterns of the HA and HA/6CS coatings (d).
Figure 7
Figure 7
CCK-8 results representing the MC3T3-E1 cell proliferation on the different coatings, data expressed as mean ± SD (n = 5), *a statistical significance compared to the HA group (P < 0.05) (a), and cell morphologies on HA (b), HA/0.2CS (c), HA/0.5CS (d), HA/2CS (e), HA/6CS (f) after culturing for a day.
Figure 8
Figure 8
Bacterial colony of different samples for cultivated in bacteria solution for 24 hours: HA (a), blank control (b), HA/0.2CS (c), HA/0.5CS (d), HA/2CS (e), HA/6CS (f). The absorbance value representing the antibacterial effect (data was expressed as mean ± SD (n = 3), a statistical significance can be seen when compared to the control and HA groups) (g), and the ZOI test result of HA/6CS (h).
Figure 9
Figure 9
The antibacterial rate of different surface coatings after cultivated in bacteria solution for 24, 48, 96 and 144 hours (HA coating as the control sample): HA/0.2CS (a), HA/0.5CS (b), HA/1CS (c), HA/2CS (d), HA/4CS (e) and HA/6CS (f). Data expressed as mean ± SD (n = 3), *a statistical significance compared to the HA group (P < 0.05).
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