The NSL complex maintains nuclear architecture stability via lamin A/C acetylation

Nat Cell Biol. 2019 Oct;21(10):1248-1260. doi: 10.1038/s41556-019-0397-z. Epub 2019 Oct 1.


While nuclear lamina abnormalities are hallmarks of human diseases, their interplay with epigenetic regulators and precise epigenetic landscape remain poorly understood. Here, we show that loss of the lysine acetyltransferase MOF or its associated NSL-complex members KANSL2 or KANSL3 leads to a stochastic accumulation of nuclear abnormalities with genomic instability patterns including chromothripsis. SILAC-based MOF and KANSL2 acetylomes identified lamin A/C as an acetylation target of MOF. HDAC inhibition or acetylation-mimicking lamin A derivatives rescue nuclear abnormalities observed in MOF-deficient cells. Mechanistically, loss of lamin A/C acetylation resulted in its increased solubility, defective phosphorylation dynamics and impaired nuclear mechanostability. We found that nuclear abnormalities include EZH2-dependent histone H3 Lys 27 trimethylation and loss of nascent transcription. We term this altered epigenetic landscape "heterochromatin enrichment in nuclear abnormalities" (HENA). Collectively, the NSL-complex-dependent lamin A/C acetylation provides a mechanism that maintains nuclear architecture and genome integrity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Animals
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure*
  • Cells, Cultured
  • Epigenesis, Genetic
  • Fibroblasts
  • Heterochromatin
  • Histone Acetyltransferases / genetics
  • Histone Acetyltransferases / metabolism*
  • Histones / genetics
  • Histones / metabolism
  • Lamin Type A / chemistry
  • Lamin Type A / genetics
  • Lamin Type A / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*


  • Heterochromatin
  • Histones
  • KANSL1 protein, mouse
  • Lamin Type A
  • Nuclear Proteins
  • Histone Acetyltransferases
  • Kat8 protein, mouse