Cryo-electron microscopy targets in CASP13: Overview and evaluation of results

Proteins. 2019 Dec;87(12):1128-1140. doi: 10.1002/prot.25817. Epub 2019 Oct 23.


Structures of seven CASP13 targets were determined using cryo-electron microscopy (cryo-EM) technique with resolution between 3.0 and 4.0 Å. We provide an overview of the experimentally derived structures and describe results of the numerical evaluation of the submitted models. The evaluation is carried out by comparing coordinates of models to those of reference structures (CASP-style evaluation), as well as checking goodness-of-fit of modeled structures to the cryo-EM density maps. The performance of contributing research groups in the CASP-style evaluation is measured in terms of backbone accuracy, all-atom local geometry and similarity of inter-subunit interfaces. The results on the cryo-EM targets are compared with those on the whole set of eighty CASP13 targets. A posteriori refinement of the best models in their corresponding cryo-EM density maps resulted in structures that are very close to the reference structure, including some regions with better fit to the density.

Keywords: CASP; cryo-EM; electron microscopy; model evaluation; protein structure prediction.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryoelectron Microscopy
  • Models, Molecular
  • Protein Conformation*
  • Proteins / chemistry
  • Proteins / genetics
  • Proteins / ultrastructure*


  • Proteins