A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PC R

Methods Mol Biol. 2020:2065:55-64. doi: 10.1007/978-1-4939-9833-3_5.


The levels of expression of the HLA-class I molecules are critical for modulating T/NK lymphocytes effector functions. Among HLA molecules, HLA-C, the most recent developed form of class I antigens, is subjected to multiple post transcriptional level of regulation that affect its cell surface expression.We describe a new method of allele-specific real-time PCR that monitor the integrity/disruption of the binding site of the microRNA Hsa-miR-148a, a key factor associated to the levels of HLA-C expression in the Caucasian populations.

Keywords: Allele-specific real-time PCR; Epigenetic regulation; HLA-C expression; Single-nucleotide polymorphism; miRNA 148a binding site.

MeSH terms

  • Alleles
  • Binding Sites / genetics
  • DNA / genetics
  • DNA / isolation & purification
  • Gene Expression Regulation / immunology
  • HLA-C Antigens / genetics*
  • HLA-C Antigens / immunology
  • Healthy Volunteers
  • Humans
  • Leukocytes, Mononuclear
  • MicroRNAs / metabolism*
  • Real-Time Polymerase Chain Reaction / methods*
  • White People / genetics


  • HLA-C Antigens
  • MIRN148 microRNA, human
  • MicroRNAs
  • DNA