Visualization of ultraviolet absorption distribution beyond the diffraction limit of light by electron-beam excitation-assisted optical microscope

W Inami; M Fukuta; Y Kawata; S Terakawa

doi:10.1111/jmi.12835

Visualization of ultraviolet absorption distribution beyond the diffraction limit of light by electron-beam excitation-assisted optical microscope

J Microsc. 2019 Oct;276(1):46-50. doi: 10.1111/jmi.12835. Epub 2019 Oct 9.

Authors

W Inami^{1

2}, M Fukuta¹, Y Kawata^{1

2}, S Terakawa³

Affiliations

¹ Graduate School of Science and Technology, Shizuoka University, Hamamatsu, Japan.
² Research Institute of Electronics, Shizuoka University, Hamamatsu, Japan.
³ Tokoha University, Shizuoka, Japan.

PMID: 31578711
DOI: 10.1111/jmi.12835

Abstract

We demonstrated that the high spatial resolution absorption contrast imaging of the crystal of vitamin B9 has absorption at ultraviolet wavelengths. The absorption wavelength matches with the wavelength of the emission of the fluorescent thin film of an electron-beam excitation-assisted (EXA) optical microscope. The fine crystal structure was imaged beyond the optical diffraction limit. The image contrast corresponded with the thickness of the crystal. The illumination light is absorbed with the vitamin B9 crystal and the intensity of the transmitted light depends on the thickness of the vitamin B9 crystal. The EXA optical microscope is useful for analysis of growth of a crystal, bioimaging and so on.

Keywords: Absorption imaging; near-field microscopy; super-resolution.