Tracking of GFP-labeled unrestricted somatic stem cells transplanted in the sepsis mouse model

Tissue Cell. 2019 Oct:60:33-37. doi: 10.1016/j.tice.2019.07.009. Epub 2019 Jul 31.

Abstract

Cell-based therapy provides a promising approach for the treatment of sepsis and related disorders. Fate determination of transplanted cells is the most essential issue for cell therapist. Optical imaging is the reliable, time and cost-effective system for cell tracking. The present study was aimed to apply an optical imaging system for monitoring of GFP-labeled unrestricted somatic stem cells in the sepsis animal model. In vivo imaging showed the most accumulation of intravenously injected cells into the lungs and liver of septic mice. Thereafter, the imaging data were more approved by flow cytometry and immunohistochemistry staining. Cellular localization in septic lungs and liver that observed by optical imaging technique may offer beneficial evidence for designing of sepsis clinical trials.

Keywords: Cell tracking; Cell transplantation; Optical imaging; Sepsis; Umbilical cord blood.

MeSH terms

  • Adult Stem Cells*
  • Animals
  • Disease Models, Animal
  • Genes, Reporter
  • Green Fluorescent Proteins*
  • Intravital Microscopy
  • Mice
  • Mice, Inbred C57BL
  • Sepsis / blood
  • Sepsis / diagnostic imaging*
  • Sepsis / therapy
  • Stem Cell Transplantation

Substances

  • Green Fluorescent Proteins