GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126

Development. 2019 Nov 5;146(21):dev184218. doi: 10.1242/dev.184218.


Mutations in the transcription factor GATA2 cause lymphedema. GATA2 is necessary for the development of lymphatic valves and lymphovenous valves, and for the patterning of lymphatic vessels. Here, we report that GATA2 is not necessary for valvular endothelial cell (VEC) differentiation. Instead, GATA2 is required for VEC maintenance and morphogenesis. GATA2 is also necessary for the expression of the cell junction molecules VE-cadherin and claudin 5 in lymphatic vessels. We identified miR-126 as a target of GATA2, and miR-126-/- embryos recapitulate the phenotypes of mice lacking GATA2. Primary human lymphatic endothelial cells (HLECs) lacking GATA2 (HLECΔGATA2) have altered expression of claudin 5 and VE-cadherin, and blocking miR-126 activity in HLECs phenocopies these changes in expression. Importantly, overexpression of miR-126 in HLECΔGATA2 significantly rescues the cell junction defects. Thus, our work defines a new mechanism of GATA2 activity and uncovers miR-126 as a novel regulator of mammalian lymphatic vascular development.

Keywords: Claudin 5; GATA2; Lymphatic vasculature; Lymphovenous valves; VE-cadherin; miR-126.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiopoietin-2 / metabolism
  • Animals
  • CRISPR-Cas Systems
  • Calcium-Binding Proteins / metabolism
  • Cell Differentiation
  • Cell Line
  • Claudin-5 / metabolism
  • EGF Family of Proteins / metabolism
  • Endothelial Cells / metabolism*
  • Endothelium, Vascular / metabolism
  • Female
  • GATA2 Transcription Factor / metabolism*
  • Gene Deletion
  • Humans
  • Lymphatic Vessels / cytology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • MicroRNAs / metabolism*
  • Mutation*
  • RNA-Seq


  • ANGPT2 protein, human
  • Angiopoietin-2
  • Angpt2 protein, mouse
  • CLDN5 protein, human
  • Calcium-Binding Proteins
  • Claudin-5
  • Cldn5 protein, mouse
  • EGF Family of Proteins
  • EGFL7 protein, human
  • Egfl7 protein, mouse
  • GATA2 Transcription Factor
  • GATA2 protein, human
  • Gata2 protein, mouse
  • MIRN126 microRNA, human
  • MIRN126 microRNA, mouse
  • MicroRNAs

Associated data

  • Dryad/10.5061/dryad.3st9688