Introduction: Genetic factors and lifestyle exposures, as well as their combinations, play important roles in the development of hypertension. We examined whether cigarette smoking, alcohol drinking and the Glu298Asp polymorphism of the endothelial nitric oxide synthase (eNOS) gene generate combined effects on blood pressure (BP) in hypertensive subjects.
Methods: A total of 342 essential hypertensive subjects were recruited from Susong community in Anhui province, China, from July 2017 to January 2018, and the plasma biochemical parameters and the genotype on Glu298Asp polymorphism were determined.
Results: There were no gender differences in the distributions of alleles and genotypes in hypertensive subjects. The proportions of cigarette smoking and alcohol drinking in male hypertensive subjects were remarkably higher than those in the females (p<0.001). The systolic blood pressure (SBP) and diastolic blood pressure (DBP) levels of mutant genotypes (Glu/Asp and Asp/Asp) were significantly higher than those of wild genotype (Glu/Glu) (p=0.013 and 0.026, respectively) in male hypertensive subjects. Moreover, the SBP and DBP levels of the mutant genotype were remarkably higher than those of wild genotype in both cigarette smoking and alcohol drinking male hypertensive subjects (p=0.034 and 0.043, respectively).
Conclusions: Cigarette smoking, alcohol drinking and the Glu298Asp polymorphism of the eNOS gene generate combined effects that increase the susceptibility of the mutant genotype to BP in Chinese male hypertensive subjects.
Keywords: Glu298Asp polymorphism; alcohol drinking; blood pressure; cigarette smoking; combined effect.
© 2019 Hong Z.
Conflict of interest statement
The authors have completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest and none was reported.
Endothelial nitric oxide synthase gene Glu298Asp polymorphism and blood pressure, left ventricular mass and carotid artery atherosclerosis in a population-based cohort.J Intern Med. 2002 Feb;251(2):102-10. doi: 10.1046/j.1365-2796.2002.00933.x. J Intern Med. 2002. PMID: 11905585
Association analyses between genetic polymorphisms of endothelial nitric oxide synthase gene and hypertension in Japanese: The Suita Study.J Hypertens. 2001 Nov;19(11):1941-8. doi: 10.1097/00004872-200111000-00003. J Hypertens. 2001. PMID: 11677358
[Effects of G894T mutation in the endothelial nitric oxide synthase gene on blood pressure].Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Jan;24(1):36-9. Zhonghua Liu Xing Bing Xue Za Zhi. 2003. PMID: 12678961 Chinese.
Endothelial nitric oxide synthase Glu298Asp gene polymorphism is associated with hypertensive response to exercise in well-controlled hypertensive patients.Yonsei Med J. 2007 Jun 30;48(3):389-395. doi: 10.3349/ymj.2007.48.3.389. Yonsei Med J. 2007. PMID: 17594145 Free PMC article.
Association between endothelial nitric oxide synthase Glu298Asp gene polymorphism and diabetic nephropathy susceptibility.Ren Fail. 2013;35(1):173-8. doi: 10.3109/0886022X.2012.734939. Epub 2012 Nov 1. Ren Fail. 2013. PMID: 23113598 Review.