Histamine H4 receptor regulates IL-6 and INF-γ secretion in native monocytes from healthy subjects and patients with allergic rhinitis

Hua Peng; Jian Wang; Xiao Yan Ye; Jie Cheng; Cheng Zhi Huang; Li Yue Li; Tian Ying Li; Chun Wei Li

doi:10.1186/s13601-019-0288-1

Histamine H4 receptor regulates IL-6 and INF-γ secretion in native monocytes from healthy subjects and patients with allergic rhinitis

Clin Transl Allergy. 2019 Sep 30:9:49. doi: 10.1186/s13601-019-0288-1. eCollection 2019.

Authors

Hua Peng^#¹, Jian Wang^#¹, Xiao Yan Ye^{2

3}, Jie Cheng¹, Cheng Zhi Huang¹, Li Yue Li^{2

3}, Tian Ying Li^{2

3}, Chun Wei Li^{2

3}

Affiliations

¹ 1Department of Otorhinolaryngology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, China.
² 2Department of Otolaryngology, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhong Shan 2nd Street, Guangzhou, 510080 China.
³ 3Guangzhou Key Laboratory of Otorhinolaryngology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

^# Contributed equally.

Abstract

Histamine H1 receptor (H1R) and histamine H4 receptor (H4R) are essential in allergic inflammation. The roles of H4R have been characterized in T cell subsets, whereas the functional properties of H4R in monocytes remain unclear. In the current study, the responses of H4R in peripheral monocytes from patients with allergic rhinitis (AR) were investigated. The results confirmed that H4R has the functional effects of mediating cytokine production (i.e., down-regulating IFN-γ and up-regulating IL-6) in cells from a monocyte cell line following challenge with histamine. We demonstrated that when monocytes from AR patients were stimulated with allergen extracts of house dust mite (HDM), IFN-γ secretion was dependent on H4R activity, but IL-6 secretion was based on H1R activity. Furthermore, a combination of H1R and H4R antagonists was more effective at blocking the inflammatory response in monocytes than treatment with either type of antagonist alone.

Keywords: Allergic rhinitis; Histamine H1 receptor; Histamine H4 receptor; Native human monocyte; Th1 and Th2 cytokines.