Plasmid conjugation is intimately linked to the transmission of antibiotic resistances, and many naturally occurring plasmids carry antibiotic resistance genes. Here we describe classical methods based on the transmission of antibiotic resistance determinants routinely used to quantify plasmid conjugation under laboratory conditions. Methods described here are suitable for most plasmid incompatibility groups from Proteobacteria and can be readily adapted to other bacterial species.
Keywords: Antibiotic resistance; Bacterial conjugation; Plasmid conjugation.