Lysobacter oculi sp. nov., isolated from human Meibomian gland secretions

Antonie Van Leeuwenhoek. 2020 Jan;113(1):13-20. doi: 10.1007/s10482-019-01289-1. Epub 2019 Oct 5.

Abstract

A Gram-stain negative, rod-shaped bacterial, catalase and oxidase positive strain (83-4T) that formed yellow colonies was isolated from human Meibomian gland secretions. Strain 83-4T belongs to the genus Lysobacter according to phylogenetic analysis based on 16S rRNA gene sequences. The DNA G+C content was 67.1 mol%. The circular genome was 2.6 Mb, which contained 2431 protein-coding sequences, 75 pseudogenes, 46 tRNAs, 3 rRNAs and 4 ncRNAs. A bacteriocin cluster and aryl polyene cluster were also found in the genome. The average nucleotide identity value was 79.6% between isolate 83-4T and the closely related type strain Lysobacter tolerans UM1T. The estimated DNA-DNA hybridization value between strain 83-4T and L. tolerans UM1T was 41.6%. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. Iso-C15:0, iso-C11:0 3-OH, iso-C11:0 and summed feature 9 (iso-C17:1ω9c) were the major fatty acids. Ubiquinone (Q-8) was the only respiratory quinone. Therefore, based on the data of phylogenetic analysis, chemotaxonomical and biochemical analyses, it is concluded that strain 83-4T represents a novel species of the genus Lysobacter with the name of Lysobacter oculi sp. nov. The type strain is 83-4T (= CGMCC 1.13464T = NRBC 113451T).

Keywords: 16S rRNA gene; Biochemical analyses; Lysobacter; Novel species.

MeSH terms

  • Base Composition / genetics
  • Cardiolipins / metabolism
  • DNA, Bacterial / genetics*
  • Humans
  • Lysobacter / classification*
  • Lysobacter / genetics*
  • Lysobacter / metabolism
  • Meibomian Glands / microbiology*
  • Phosphatidylethanolamines / metabolism
  • Phylogeny
  • Pseudogenes / genetics
  • RNA, Ribosomal, 16S / genetics

Substances

  • Cardiolipins
  • DNA, Bacterial
  • Phosphatidylethanolamines
  • RNA, Ribosomal, 16S
  • phosphatidylethanolamine