Enhanced dual network hydrogels consisting of thiolated chitosan and silk fibroin for cartilage tissue engineering

Abstract

Thiolated chitosan (CS-NAC) was synthesized and the selected CS-NAC was used together with silk fibroin (SF) to produce dual network CS-NAC/SF hydrogels. The CS-NAC/SF solutions with formulated compositions were able to form hydrogels at physiological temperature and pH. Rheological measurements showed that elastic modulus of some CS-NAC/SF gels could reach around 3 kPa or higher and was much higher than their respective viscous modulus, indicating that they behaved like strong gels. Deformation measurements verified that CS-NAC/SF gels had well-defined elasticity. The optimized CS-NAC/SF gels exhibited jointly enhanced properties in terms of strength, stiffness and elasticity when compared to the gels resulted from either CS-NAC or SF. Examinations of dry CS-NAC/SF gels revealed that they were highly porous with well-interconnected pore features. Cell culture demonstrated that CS-NAC/SF gels supported the growth of chondrocytes while effectively maintaining their phenotype. Results suggest that these dual network gels have promising potential in cartilage repair.

Keywords: Cartilage tissue engineering; Dual network structure; Hydrogel; Silk fibroin; Thiolated chitosan.