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. 2019 Nov;29(11):956-959.
doi: 10.1038/s41422-019-0238-4. Epub 2019 Oct 7.

Precise temporal regulation of Dux is important for embryo development

Mingyue Guo #  1 , Yanping Zhang #  1 , Jianfeng Zhou  1 , Yan Bi  1 , Junqin Xu  1 , Ce Xu  1 , Xiaochen Kou  1 , Yanhong Zhao  1 , Yanhe Li  1 , Zhifen Tu  1 , Kuisheng Liu  1 , Jiaming Lin  1 , Peng Yang  2 , Shaorong Gao  3 , Yixuan Wang  4
Affiliations

Precise temporal regulation of Dux is important for embryo development

Mingyue Guo et al. Cell Res. 2019 Nov.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
a Schematic overview of Dux cluster locus in WT and Dux-KO mice, and distribution of third-generation long-read (30 kb) at the Dux cluster of Dux-KO mice. Red and blue arrowheads indicate targeting sites of sgRNAs. b Statistical analysis of litter size generated by WT, Dux-Het and Dux-KO mice. Each dot represents a single litter analyzed. ***P < 0.001; two-tailed Student’s t-test. Center lines and error bars indicate means and SD, respectively. c Principal component analysis of WT and Dux-KO embryos from zygotic stage to 2C stage. e-2C, early 2C stage; m-2C, middle 2C stage; l-2C, late 2C stage. d Scatter plots of gene expression (left panel) and transposable element (TE) expression (right panel) in WT and Dux-KO embryos at early 2C (e-2C) stage. FC > 2, FDR < 0.05. e Box plots of expression of 588 downregulated ZGA genes in WT and Dux-KO embryos from early to late 2C stages. e-2C, early 2C stage; m-2C, middle 2C stage; l-2C, late 2C stage. ***P < 0.001; Mann–Whitney–Wilcoxon two-sided test. The middle lines in the boxes represent medians. Box hinges indicate the twenty-fifth and seventy-fifth percentiles, and the whiskers indicate the hinge ± 1.5 × interquartile range. f Dot plots of MERVL-int expression in WT and Dux-KO embryos from zygotic to late 2C stages. Each dot represents an embryo. Zy, zygotic stage; e-2C, early 2C stage; m-2C, middle 2C stage; l-2C, late 2C stage. ***P < 0.001; two-tailed Student’s t-test. Center lines and error bars indicate means and SD, respectively. g Development progression of embryos 4 h, 8 h, and 41 h after mRNA injection in 2 blastomeres of 2C embryos. h hours, Dux-OE group with mRNA mixture of Dux-EGFP and RFP (left panels), Ctrl control group with mRNA mixture of EGFP and RFP (right panels). Scale bars, 50 μm. h Bar graph comparing the percentage of embryo at different developmental stages between Dux-OE and control groups 65 h after mRNA injection. *P < 0.05, **P < 0.01; two-tailed Student’s t-test. Error bars indicate SD. i Immunofluorescent staining of Dux-OE (upper panels) and control (lower panels) embryos with 2-blastomere injection at 3C or 4C stage. Scale bars, 10 μm. j Immunofluorescent staining of 1-blastomere-injected Dux-OE embryos at blastocyst stage. White dotted circles indicate the arrested blastomeres. Scale bars, 10 μm. k Dot plots showing expression of Dux (upper panel) and MERVL-int (bottom panel) in Dux-OE and control embryos at early and late 4C stages. Each dot represents an injected embryo. e-4C, early 4C stage; l-4C, late 4C stage. ***P < 0.001; two-tailed Student’s t-test. Center lines and error bars indicate means and SD, respectively. l Scatter plots of gene expression (left panel) and TE expression (right panel) in Dux-OE and control embryos at late 4C stage. FC > 2, FDR < 0.05. m Statistics of Dux-EGFP expression hours in MG132-treated and -untreated embryos. *P < 0.05, **P < 0.01, ***P < 0.001; two-tailed Student’s t-test. Error bars indicate SD
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