Updated guidelines for predictive biomarker testing in advanced non-small-cell lung cancer: a National Consensus of the Spanish Society of Pathology and the Spanish Society of Medical Oncology

Clin Transl Oncol. 2020 Jul;22(7):989-1003. doi: 10.1007/s12094-019-02218-4. Epub 2019 Oct 9.


In 2011 the Spanish Society of Medical Oncology (SEOM) and the Spanish Society of Pathology (SEAP) started a joint project to establish guidelines on biomarker testing in patients with advanced non-small-cell lung cancer (NSCLC) based on current evidence. As this field is constantly evolving, these guidelines have been updated, previously in 2012 and 2015 and now in 2019. Current evidence suggests that the mandatory tests to conduct in all patients with advanced NSCLC are for EGFR and BRAF mutations, ALK and ROS1 rearrangements and PD-L1 expression. The growing need to study other emerging biomarkers has promoted the routine use of massive sequencing (next-generation sequencing, NGS). The coordination of every professional involved and the prioritisation of the most suitable tests and technologies for each case remains a challenge.

Keywords: ALK; BRAF; Biomarkers; EGFR; Non-small-cell lung cancer; PD-L1; ROS1.

Publication types

  • Practice Guideline

MeSH terms

  • Anaplastic Lymphoma Kinase / genetics
  • B7-H1 Antigen / genetics
  • Biomarkers, Tumor / genetics*
  • Carcinoma, Non-Small-Cell Lung / diagnosis*
  • Carcinoma, Non-Small-Cell Lung / genetics
  • Circulating Tumor DNA
  • ErbB Receptors / genetics
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Liquid Biopsy
  • Lung Neoplasms / diagnosis*
  • Lung Neoplasms / genetics
  • Membrane Glycoproteins / genetics
  • Neoplastic Cells, Circulating
  • Polymerase Chain Reaction
  • Protein-Tyrosine Kinases / genetics
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins B-raf / genetics
  • Proto-Oncogene Proteins c-met / genetics
  • Proto-Oncogene Proteins c-ret / genetics
  • Receptor, ErbB-2 / genetics
  • Receptor, trkA / genetics
  • Receptor, trkB / genetics
  • Receptor, trkC / genetics
  • Societies, Medical
  • Spain


  • B7-H1 Antigen
  • Biomarkers, Tumor
  • CD274 protein, human
  • Circulating Tumor DNA
  • Membrane Glycoproteins
  • NTRK1 protein, human
  • NTRK3 protein, human
  • Proto-Oncogene Proteins
  • ALK protein, human
  • Anaplastic Lymphoma Kinase
  • EGFR protein, human
  • ERBB2 protein, human
  • ErbB Receptors
  • MET protein, human
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins c-met
  • Proto-Oncogene Proteins c-ret
  • RET protein, human
  • ROS1 protein, human
  • Receptor, ErbB-2
  • Receptor, trkA
  • Receptor, trkB
  • Receptor, trkC
  • tropomyosin-related kinase-B, human
  • BRAF protein, human
  • Proto-Oncogene Proteins B-raf