Prognostic plasma protein panel for Aβ deposition in the brain in Alzheimer's disease

Prog Neurobiol. 2019 Dec;183:101690. doi: 10.1016/j.pneurobio.2019.101690. Epub 2019 Oct 9.


Alzheimer's disease (AD) is the most common age-associated dementia. Many studies have sought to predict cerebral amyloid deposition, the major pathological hallmark of AD, using body fluids such as blood or cerebral spinal fluid (CSF). The use of blood in diagnostic procedures is widespread in medicine; however, existing blood biomarkers for AD remain unreliable. We sought to discover blood biomarkers that discriminate Aβ deposition status in the brain. This study used 107 individuals who were cognitively normal (CN), 107 patients with mild cognitive impairment (MCI), and 40 AD patients with Pittsburg compound B positron emission tomography (PiB-PET) amyloid imaging data available. We found five plasma biomarker candidates via mass spectrometry (MS) based-proteomic analysis and validated these proteins using enzyme-linked immunosorbent assay (ELISA). Our integrated models were highly predictive of brain amyloid deposition, exhibiting 0.871 accuracy with 79% sensitivity and 84% specificity overall, and 0.836 accuracy with 68% sensitivity and 90% specificity in patients with MCI. These results indicated that a combination of proteomic-based blood proteins might be a possible biomarker set for predicting cerebral amyloid deposition.

Keywords: Alzheimer’s disease; Cerebral amyloid deposition; PiB-PET; Plasma biomarker; Proteomics; TMT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Alzheimer Disease / blood
  • Alzheimer Disease / diagnosis*
  • Alzheimer Disease / diagnostic imaging
  • Alzheimer Disease / metabolism*
  • Amyloid beta-Peptides / metabolism*
  • Aniline Compounds
  • Biomarkers / blood*
  • Blood Chemical Analysis / standards*
  • Blood Proteins / metabolism*
  • Cognitive Dysfunction / blood
  • Cognitive Dysfunction / diagnosis*
  • Cognitive Dysfunction / diagnostic imaging
  • Cognitive Dysfunction / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Male
  • Positron-Emission Tomography / standards*
  • Predictive Value of Tests
  • Prognosis
  • Proteomics
  • Sensitivity and Specificity
  • Thiazoles


  • 2-(4'-(methylamino)phenyl)-6-hydroxybenzothiazole
  • Amyloid beta-Peptides
  • Aniline Compounds
  • Biomarkers
  • Blood Proteins
  • Thiazoles