Subacute feeding toxicity of low-sodium sausages manufactured with sodium substitutes and biopolymer-encapsulated saltwort (Salicornia herbacea) in a mouse model

J Sci Food Agric. 2020 Jan 30;100(2):794-802. doi: 10.1002/jsfa.10087. Epub 2019 Nov 14.

Abstract

Background: Low-sodium sausages were manufactured using sodium substitution and biopolymer encapsulation. A diet comprising 10% treatment sausages (six treatment groups: C (100% NaCl), T1 (55% sodium substitute + 45% saltwort salt), T2 (55% sodium substitute + 45% saltwort salt with chitosan), T3 (55% sodium substitute + 45% saltwort salt with cellulose), T4 (55% sodium substitute + 45% saltwort salt with dextrin), and T5 (55% sodium substitute + 45% saltwort salt with pectin)) was added to a 90% commercial mouse diet for 4 weeks.

Results: Subacute toxicity, hematology, liver function, and organ weight tests in low-sodium sausage groups showed results similar to those of the control group, and all toxicity test levels were within normal ranges.

Conclusions: All low-sodium sausage types tested are suggested to be safe in terms of subacute toxicity. Moreover, low-sodium sausages can be manufactured by biopolymer encapsulation of saltwort using pectin, chitosan, cellulose, and dextrin without toxicity. © 2019 Society of Chemical Industry.

Keywords: biopolymer; encapsulation; low-sodium sausage; mice; sodium substitute; subacute toxicity.

MeSH terms

  • Animals
  • Biopolymers / analysis*
  • Biopolymers / metabolism
  • Biopolymers / toxicity
  • Cellulose / analysis
  • Cellulose / metabolism
  • Cellulose / toxicity
  • Chitosan / analysis
  • Chitosan / metabolism
  • Chitosan / toxicity
  • Female
  • Food Additives / analysis*
  • Food Additives / metabolism
  • Food Additives / toxicity
  • Food Handling / instrumentation
  • Food Handling / methods*
  • Male
  • Meat Products / analysis*
  • Meat Products / toxicity
  • Mice
  • Mice, Inbred ICR
  • Salsola / chemistry*
  • Salsola / metabolism
  • Salsola / toxicity
  • Sodium / analysis*
  • Sodium / metabolism
  • Sodium / toxicity
  • Swine

Substances

  • Biopolymers
  • Food Additives
  • Cellulose
  • Chitosan
  • Sodium