Inactivation of tissue inhibitor of metalloproteinases by neutrophil elastase and other serine proteinases

FEBS Lett. 1988 Feb 29;229(1):157-60. doi: 10.1016/0014-5793(88)80817-2.

Abstract

Tissue inhibitor of metalloproteinases (TIMP) from cultured bovine dental pulp inhibits human rheumatoid synovial matrix metalloproteinase 3 (MMP-3) with a stoichiometry of 1:1 on a molar basis. Among the serine proteinases examined, human neutrophil elastase, trypsin and alpha-chymotrypsin destroyed the inhibitory activity of TIMP against MMP-3 by degrading the inhibitor molecule into small fragments. In contrast, the inhibitory activity of TIMP was not significantly reduced by the actions of cathepsin G, pancreatic elastase and plasmin. These data indicate that neutrophils which infiltrate tissues in various inflammatory conditions may play an important role in regulating TIMP activity in vivo through the action of neutrophil elastase.

MeSH terms

  • Animals
  • Arthritis, Rheumatoid / enzymology
  • Cattle
  • Chymotrypsin / metabolism
  • Enzyme Inhibitors / pharmacology*
  • Humans
  • Metalloendopeptidases / antagonists & inhibitors*
  • Neutrophils / enzymology*
  • Pancreatic Elastase / blood*
  • Serine Endopeptidases / blood*
  • Synovial Membrane / enzymology
  • Tissue Inhibitor of Metalloproteinases
  • Trypsin / metabolism

Substances

  • Enzyme Inhibitors
  • Tissue Inhibitor of Metalloproteinases
  • Serine Endopeptidases
  • Chymotrypsin
  • Pancreatic Elastase
  • Trypsin
  • Metalloendopeptidases