Chitosan-based nitric oxide-releasing dressing for anti-biofilm and in vivo healing activities in MRSA biofilm-infected wounds

Int J Biol Macromol. 2020 Jan 1:142:680-692. doi: 10.1016/j.ijbiomac.2019.10.009. Epub 2019 Oct 14.

Abstract

Bacterial biofilms on wounds impair the healing process and often lead to chronic wounds. Chitosan is a well-known biopolymer with antimicrobial and anti-biofilm effects. S-nitrosoglutathione (GSNO) has been identified as a promising nitric oxide (NO) donor to defend against pathogenic biofilms and enhance wound healing activities. In this study, we prepared NO-releasing chitosan film (CS/NO film) and evaluated its anti-biofilm activity and in vivo wound healing efficacy against methicillin-resistant Staphylococcus aureus (MRSA) biofilm-infected wounds in diabetic mice. The in vitro release study showed sustained release of NO over 3 days in simulated wound fluid. The CS/NO film significantly enhanced antibacterial activity against MRSA by > 3 logs reduction in bacterial viability. Moreover, CS/NO film exhibited a 3-fold higher anti-biofilm activity than the control and CS film. In in vivo MRSA biofilm-infected wounds, the CS/NO film-treated group showed faster biofilm dispersal, wound size reduction, epithelialization rates, and collagen deposition than the untreated and CS film-treated groups. Therefore, the CS/NO film investigated in this study could be a promising approach for the treatment of MRSA biofilm-infected wounds.

Keywords: Chitosan dressing; MRSA biofilm-infected wound healing; Nitric oxide-releasing system.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology
  • Bandages
  • Biofilms
  • Cell Line
  • Cell Survival
  • Chitosan / chemistry*
  • Collagen / drug effects
  • Diabetes Mellitus, Experimental
  • Drug Delivery Systems
  • Drug Liberation
  • Hydrogels / chemistry
  • Male
  • Methicillin-Resistant Staphylococcus aureus / drug effects
  • Mice
  • Mice, Inbred ICR
  • Nitric Oxide / chemistry*
  • Nitric Oxide / pharmacology
  • Wound Healing / drug effects*

Substances

  • Anti-Bacterial Agents
  • Hydrogels
  • Nitric Oxide
  • Collagen
  • Chitosan