Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning

Methods Mol Biol. 2020:2070:157-171. doi: 10.1007/978-1-4939-9853-1_9.


Affinity selection using phage-display technologies is a powerful tool for identifying the peptide epitopes of monoclonal antibodies. Coupling affinity selection with deep sequencing technologies allows for the broad assessment of selectant populations. Here, we describe a method for using a phage-display platform to assess antibody specificity in human serum. We describe the method with reference to the bacteriophage MS2 virus-like particle (VLP) platform, but it can be adapted to other phage-display technologies as well.

Keywords: Affinity selection; Antibodies; Deep sequencing; Pathogens; Phage display; Serum; Virus-like particle.

MeSH terms

  • Antibody Specificity*
  • Epitopes / chemistry*
  • Humans
  • Levivirus* / chemistry
  • Levivirus* / genetics
  • Peptide Library*
  • Serum / chemistry*
  • Single-Chain Antibodies* / chemistry
  • Single-Chain Antibodies* / genetics


  • Epitopes
  • Peptide Library
  • Single-Chain Antibodies