Is Human Papillomavirus and Helicobacter pylori Related in Gastric Lesions?

Clin Lab. 2019 Oct 1;65(10). doi: 10.7754/Clin.Lab.2019.181244.


Background: Human papillomavirus (HPV), the causative agent of cervical cancer, is also suggested as a risk factor for gastric adenocarcinoma. Many infectious agents besides Helicobacter pylori have been associated with gastritis. The aim of this study was to investigate HPV DNA and genotyping HPV type 16 DNA in gastric adenocarcinoma and Helicobacter pylori gastritis cases.

Methods: A hundred and six gastric adenocarcinoma and Helicobacter pylori gastritis samples and 26 controls were included. After deparaffinization by xylene, DNA extraction was performed by the phenol-chloroform-isoamyl alcohol method and 106 samples were studied with a G6PDH control kit (Eurogentec, Seraing, Belgium). Fifty-three adenocarcinoma and 43 Helicobacter pylori samples were thought to have enough tissue and were studied for HPV DNA. HPV types other than 16 and HPV type 16 DNA were detected by Real Time PCR using the L1 region. Amplifications of MY09/11 products were done by GP5+/GP6+ primers and Cyanine-5 labeled HPV DNA and HPV 16 DNA specific probe in Light Cycler 2.0 (Roche Diagnostics, Germany) device.

Results: Among gastric adenocarcinoma and Helicobacter pylori gastritis samples, 20/53 (38%) and 18/43 (41.8%) were HPV DNA positive, respectively. Five (19.2%) of 26 controls were HPV DNA positive.

Conclusions: Our 38% positive result in the gastric carcinoma group is in concordance with previous reports. This is the first study revealing the HPV-H. pylori relationship in gastritis cases and we concluded that with regard to the nearly three-fold higher HPV DNA (41.8%) in gastritis cases compared to controls, Helicobacter pylori positive cases should also be evaluated in favor of HPV in the gastritis group.

MeSH terms

  • Adenocarcinoma / diagnosis*
  • Adenocarcinoma / microbiology
  • Adenocarcinoma / virology
  • Adult
  • Aged
  • DNA, Viral / genetics
  • Female
  • Gastritis / diagnosis*
  • Gastritis / microbiology
  • Gastritis / virology
  • Genotype
  • Helicobacter Infections / diagnosis*
  • Helicobacter Infections / microbiology
  • Helicobacter Infections / virology
  • Helicobacter pylori / physiology
  • Human papillomavirus 16 / genetics
  • Human papillomavirus 16 / physiology
  • Humans
  • Male
  • Middle Aged
  • Papillomaviridae / genetics*
  • Papillomaviridae / physiology
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / virology
  • Retrospective Studies
  • Stomach Neoplasms / diagnosis*
  • Stomach Neoplasms / microbiology
  • Stomach Neoplasms / virology


  • DNA, Viral