Quantitative study of doxorubicin in living cell nuclei by microspectrofluorometry

Biochim Biophys Acta. 1988 May 6;950(1):13-20. doi: 10.1016/0167-4781(88)90068-1.


Doxorubicin-DNA association has been studied by quantitative microspectrofluorometry. Fluorescence emission spectra from a microvolume of single living cell nuclei treated with doxorubicin have been analyzed in terms of difference in spectral shape and fluorescence yield between free and DNA-bound drug. Contribution of each spectral component to the total signal was calculated by least-squares linear regression. With this method of analysis, total drug concentration has been determined with an error of less than 10%. Moreover, the uptake into the nucleus has been studied in a non destructive way, avoiding use of 14C-labelled drug. Kinetic studies of drug accumulation into the nuclei were conducted on sensitive and resistant cells.

Publication types

  • Comparative Study

MeSH terms

  • Cell Nucleus / analysis*
  • DNA, Neoplasm / analysis
  • Doxorubicin / analysis*
  • Drug Resistance
  • Humans
  • Leukemia, Erythroblastic, Acute / pathology
  • Microchemistry
  • Spectrometry, Fluorescence
  • Tumor Cells, Cultured / analysis
  • Tumor Cells, Cultured / drug effects


  • DNA, Neoplasm
  • Doxorubicin