Effect of Quorum Sensing on the Ability of Desulfovibrio vulgaris To Form Biofilms and To Biocorrode Carbon Steel in Saline Conditions

Appl Environ Microbiol. 2019 Dec 13;86(1):e01664-19. doi: 10.1128/AEM.01664-19. Print 2019 Dec 13.

Abstract

Sulfate-reducing bacteria (SRB) are key contributors to microbe-induced corrosion (MIC), which can lead to serious economic and environmental impact. The presence of a biofilm significantly increases the MIC rate. Inhibition of the quorum-sensing (QS) system is a promising alternative approach to prevent biofilm formation in various industrial settings, especially considering the significant ecological impact of conventional chemical-based mitigation strategies. In this study, the effect of the QS stimulation and inhibition on Desulfovibrio vulgaris is described in terms of anaerobic respiration, cell activity, biofilm formation, and biocorrosion of carbon steel. All these traits were repressed when bacteria were in contact with QS inhibitors but enhanced upon exposure to QS signal molecules compared to the control. The difference in the treatments was confirmed by transcriptomic analysis performed at different time points after treatment application. Genes related to lactate and pyruvate metabolism, sulfate reduction, electron transfer, and biofilm formation were downregulated upon QS inhibition. In contrast, QS stimulation led to an upregulation of the above-mentioned genes compared to the control. In summary, these results reveal the impact of QS on the activity of D. vulgaris, paving the way toward the prevention of corrosive SRB biofilm formation via QS inhibition.IMPORTANCE Sulfate-reducing bacteria (SRB) are considered key contributors to biocorrosion, particularly in saline environments. Biocorrosion imposes tremendous economic costs, and common approaches to mitigate this problem involve the use of toxic and hazardous chemicals (e.g., chlorine), which raise health and environmental safety concerns. Quorum-sensing inhibitors (QSIs) can be used as an alternative approach to inhibit biofilm formation and biocorrosion. However, this approach would only be effective if SRB rely on QS for the pathways associated with biocorrosion. These pathways would include biofilm formation, electron transfer, and metabolism. This study demonstrates the role of QS in Desulfovibrio vulgaris on the above-mentioned pathways through both phenotypic measurements and transcriptomic approach. The results of this study suggest that QSIs can be used to mitigate SRB-induced corrosion problems in ecologically sensitive areas.

Keywords: biocorrosion; biofilm; quorum sensing; sulfate-reducing bacteria; transcriptomic analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyl-Butyrolactones / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Biofilms / drug effects*
  • Biofilms / growth & development
  • Carbon / metabolism
  • Corrosion
  • Desulfovibrio vulgaris / genetics
  • Desulfovibrio vulgaris / growth & development*
  • Desulfovibrio vulgaris / metabolism
  • Energy Metabolism / genetics
  • Gene Expression Regulation
  • Genes, Bacterial
  • Lactic Acid / metabolism
  • Plankton / microbiology
  • Pyruvic Acid / metabolism
  • Quorum Sensing / drug effects*
  • Seawater / chemistry
  • Steel
  • Sulfates / metabolism
  • Transcription Factors / genetics
  • Transcriptome

Substances

  • Acyl-Butyrolactones
  • Bacterial Proteins
  • Sulfates
  • Transcription Factors
  • Steel
  • Lactic Acid
  • Carbon
  • Pyruvic Acid