Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays

J Microbiol Methods. 2019 Dec;167:105743. doi: 10.1016/j.mimet.2019.105743. Epub 2019 Oct 16.

Abstract

This study compared the chronological life span and survival of Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast CLS compared to the bottle-aged culture.

Keywords: Aeration; Calorie restriction; Chronological life span; Saccharomyces cerevisiae.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caloric Restriction
  • Microbial Viability
  • Microbiological Techniques / instrumentation
  • Microbiological Techniques / methods*
  • Saccharomyces cerevisiae / growth & development*