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. 2019 Dec;167:105743.
doi: 10.1016/j.mimet.2019.105743. Epub 2019 Oct 16.

Comparison of Microplate- And Bottle-Based Methods to Age Yeast for Chronological Life Span Assays

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Comparison of Microplate- And Bottle-Based Methods to Age Yeast for Chronological Life Span Assays

Mandy Mun Yee Kwong et al. J Microbiol Methods. .

Abstract

This study compared the chronological life span and survival of Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast CLS compared to the bottle-aged culture.

Keywords: Aeration; Calorie restriction; Chronological life span; Saccharomyces cerevisiae.

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