Insect hemocyte and fat body tissues play critical functional roles in insect immunity. Little, however, is known concerning the dynamic responses of these tissues to fungal infection. Here, we report on a time course of locust hemocyte and fat body transcriptomic responses to infection by the acridid specific fungal pathogen, Metarhizium acridum. Fat body responses were more pronounced at all infection stages as compared to hemocytes. Immune and other related genes were induced far earlier than previously considered including at pre-penetration stages. Differential expression in hemocyte and fat body tissues persisted throughout the course of infection up until host death. Our data indicate selective pressure on the host to recognize the infection as early as possible in order to limit its spread. Overall, fat body and hemocyte tissues launch a robust multi-tiered response to combat the fungal pathogen, with our data providing potential host targets for exploitation in pest control.
Keywords: Fat body; Hemocytes; Infection time course; Locust; Metarhizium; Transcriptomics.
Copyright © 2019 Elsevier Ltd. All rights reserved.
Comparative transcriptomic analysis of immune responses of the migratory locust, Locusta migratoria, to challenge by the fungal insect pathogen, Metarhizium acridum.BMC Genomics. 2015 Oct 26;16:867. doi: 10.1186/s12864-015-2089-9. BMC Genomics. 2015. PMID: 26503342 Free PMC article.
Inhibitor of apoptosis-1 gene as a potential target for pest control and its involvement in immune regulation during fungal infection.Pest Manag Sci. 2020 May;76(5):1831-1840. doi: 10.1002/ps.5712. Epub 2020 Jan 3. Pest Manag Sci. 2020. PMID: 31821720
Large scale expressed sequence tag (EST) analysis of Metarhizium acridum infecting Locusta migratoria reveals multiple strategies for fungal adaptation to the host cuticle.Curr Genet. 2012 Dec;58(5-6):265-79. doi: 10.1007/s00294-012-0382-6. Epub 2012 Oct 7. Curr Genet. 2012. PMID: 23052419
Insect immunology and hematopoiesis.Dev Comp Immunol. 2016 May;58:102-18. doi: 10.1016/j.dci.2015.12.006. Epub 2015 Dec 13. Dev Comp Immunol. 2016. PMID: 26695127 Free PMC article. Review.
Biological mediators of insect immunity.Annu Rev Entomol. 1997;42:611-43. doi: 10.1146/annurev.ento.42.1.611. Annu Rev Entomol. 1997. PMID: 9017902 Review.